Repetitive DNAs and chromosome evolution in Megaleporinus obtusidens and M. reinhardti (Characiformes: Anostomidae)

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-08 DOI:10.1007/s10709-024-00206-3

Thais Aparecida Dulz, Matheus Azambuja, Carla Andrea Lorscheider, Rafael Bueno Noleto, Orlando Moreira-Filho, Viviane Nogaroto, Viviane Demetrio Nascimento, Débora Diniz, Paulo Roberto Antunes de Mello Affonso, Marcelo Ricardo Vicari

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Abstract

The high dynamism of repetitive DNAs is a major driver of chromosome evolution. In particular, the accumulation of repetitive DNA sequences has been reported as part of the differentiation of sex-specific chromosomes. In turn, the fish species of the genus Megaleporinus are a monophyletic clade in which the presence of differentiated ZZ/ZW sex chromosomes represents a synapomorphic condition, thus serving as a suitable model to evaluate the dynamic evolution of repetitive DNA classes. Therefore, transposable elements (TEs) and in tandem repeats were isolated and located on chromosomes of Megaleporinus obtusidens and M. reinhardti to infer their role in chromosome differentiation with emphasis on sex chromosome systems. Despite the conserved karyotype features of both species, the location of repetitive sequences - Rex 1, Rex 3, (TTAGGG)_n, (GATA)_n, (GA)n, (CA)_n, and (A)_n - varied both intra and interspecifically, being mainly accumulated in Z and W chromosomes. The physical mapping of repetitive sequences confirmed the remarkable dynamics of repetitive DNA classes on sex chromosomes that might have promoted chromosome diversification and reproductive isolation in Megaleporinus species.

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Megaleporinus obtusidens和M. reinhardti（蛛形纲：Anostomidae）的重复DNA和染色体进化

重复 DNA 的高度动态性是染色体进化的主要驱动力。特别是，据报道，重复 DNA 序列的积累是性别特异性染色体分化的一部分。而 Megaleporinus 属的鱼类物种是一个单系支系，其中分化的 ZZ/ZW 性染色体的存在代表了一种同形条件，因此是评估重复 DNA 类动态进化的合适模型。因此，我们在Megaleporinus obtusidens和M. reinhardti的染色体上分离并定位了转座元件（TE）和串联重复序列，以推断它们在染色体分化中的作用，重点是性染色体系统。尽管两个物种的核型特征一致，但重复序列--Rex 1、Rex 3、(TTAGGG)n、(GATA)n、(GA)n、(CA)n和(A)n--的位置在种内和种间均有差异，主要累积在Z和W染色体上。重复序列的物理映射证实了性染色体上重复DNA类别的显著动态变化，这可能促进了Megaleporinus物种的染色体多样化和生殖隔离。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.