Differential expression of N-glycopeptides derived from serum glycoproteins in mild cognitive impairment (MCI) patients

IF 3.4 4区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Proteomics Pub Date : 2024-04-11 DOI:10.1002/pmic.202300620
Cristian D. Gutierrez Reyes, Mojgan Atashi, Mojibola Fowowe, Sherifdeen Onigbinde, Oluwatosin Daramola, David M. Lubman, Yehia Mechref
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引用次数: 0

Abstract

Mild cognitive impairment (MCI) is an early stage of memory loss that affects cognitive abilities with the aging of individuals, such as language or visual/spatial comprehension. MCI is considered a prodromal phase of more complicated neurodegenerative diseases such as Alzheimer's. Therefore, accurate diagnosis and better understanding of the disease prognosis will facilitate prevention of neurodegeneration. However, the existing diagnostic methods fail to provide precise and well-timed diagnoses, and the pathophysiology of MCI is not fully understood. Alterations of the serum N-glycoproteome expression could represent an essential contributor to the overall pathophysiology of neurodegenerative diseases and be used as a potential marker to assess MCI diagnosis using less invasive procedures. In this approach, we identified N-glycopeptides with different expressions between healthy and MCI patients from serum glycoproteins. Seven of the N-glycopeptides showed outstanding AUC values, among them the antithrombin-III Asn224 + 4-5-0-2 with an AUC value of 1.00 and a p value of 0.0004. According to proteomics and ingenuity pathway analysis (IPA), our data is in line with recent publications, and the glycoproteins carrying the identified N-sites play an important role in neurodegeneration.

轻度认知障碍(MCI)患者血清糖蛋白中 N-糖肽的表达差异
轻度认知障碍(MCI)是记忆力减退的早期阶段,会随着年龄的增长而影响认知能力,如语言或视觉/空间理解能力。MCI 被认为是阿尔茨海默氏症等更复杂的神经退行性疾病的前驱阶段。因此,准确诊断和更好地了解疾病预后将有助于预防神经变性。然而,现有的诊断方法无法提供准确和及时的诊断,而 MCI 的病理生理学也尚未完全清楚。血清 N-糖蛋白组表达的改变可能是神经退行性疾病整体病理生理学的一个重要因素,可作为一种潜在的标记物,使用侵入性较小的程序来评估 MCI 诊断。在这种方法中,我们从血清糖蛋白中发现了健康人和 MCI 患者表达不同的 N-糖肽。其中7种N-糖肽的AUC值表现突出,其中抗凝血酶-III Asn224 + 4-5-0-2的AUC值为1.00,P值为0.0004。根据蛋白质组学和巧妙通路分析(IPA),我们的数据与最近发表的文章一致,携带已鉴定 N 位点的糖蛋白在神经变性中发挥着重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Proteomics
Proteomics 生物-生化研究方法
CiteScore
6.30
自引率
5.90%
发文量
193
审稿时长
3 months
期刊介绍: PROTEOMICS is the premier international source for information on all aspects of applications and technologies, including software, in proteomics and other "omics". The journal includes but is not limited to proteomics, genomics, transcriptomics, metabolomics and lipidomics, and systems biology approaches. Papers describing novel applications of proteomics and integration of multi-omics data and approaches are especially welcome.
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