Immobilization of lipase enzyme onto herbal silver nanoparticles and examination of biochemical parameters of immobilized enzyme

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-12 DOI:10.1007/s43153-024-00460-0

Atefeh Nasiri, Marzieh Ghollasi, Khadijeh Eskandari, Elahe Darvishi

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Abstract

Nanoparticles are useful for immobilization due to their size and physical properties. The present study aimed to synthesize herbal silver nanoparticles (SNPs) to immobilize the lipase from Candida rugosa covalently on the nanoparticles as well as to examine the biochemical parameters of the immobilized enzyme. SNPs were synthesized using Cydonia oblonga leaf extract and were characterized. Lipase enzyme was immobilized on synthesized SNPs and the immobilization efficiency was calculated. The biochemical properties of immobilized and free enzymes, including the temperature effect and pH on enzymatic activity, thermal stability, storage stability, and reusability of the immobilized enzyme were specified. Electron microscopy, DLS measurements, and Raman spectroscopy confirmed the 50 nm SNPs and the immobilization of lipase enzyme on them. The efficiency of lipase enzyme immobilization on nanoparticles was estimated to be 48%. The free enzymes and immobilized enzymes had the highest activity at 37°C and 55°C, respectively. Also, the optimal pH was 7 for the free enzyme and 6 for the immobilized enzyme. A comparison of thermal and storage stability of free and immobilized enzymes suggested that immobilized enzymes had more stability and resistance than free enzymes as they also could be reused up to 12 times. The kinetic parameters of the immobilized enzyme compared to the free enzyme indicated a slight decrease in the maximum rate of the enzyme. Immobilized enzymes can be used in industries and are also very crucial for commercial use as they are cost-effective.

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将脂肪酶固定在中药银纳米粒子上并检测固定化酶的生化参数

纳米颗粒因其尺寸和物理特性而可用于固定化。本研究旨在合成中草药银纳米粒子（SNPs），以在纳米粒子上共价固定白色念珠菌脂肪酶，并检测固定化酶的生化参数。SNP 是用 Cydonia oblonga 叶提取物合成的，并对其进行了表征。脂肪酶被固定在合成的 SNPs 上，并计算了固定效率。研究了固定化酶和游离酶的生化特性，包括温度效应和 pH 值对酶活性的影响、固定化酶的热稳定性、储存稳定性和重复使用性。电子显微镜、DLS 测量和拉曼光谱证实了 50 纳米 SNP 及其上脂肪酶的固定化。脂肪酶固定在纳米颗粒上的效率估计为 48%。游离酶和固定化酶分别在 37°C 和 55°C 时活性最高。此外，游离酶的最佳 pH 值为 7，固定化酶的最佳 pH 值为 6。对游离酶和固定化酶的热稳定性和储存稳定性的比较表明，固定化酶比游离酶具有更高的稳定性和耐受性，因为它们还可以重复使用多达 12 次。与游离酶相比，固定化酶的动力学参数表明，酶的最大速率略有下降。固定化酶可用于工业，由于其成本效益高，在商业用途中也非常重要。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.