Beef cattle feedlot runoff impacts on soil antimicrobial resistance

IF 1.3 Q3 AGRONOMY
Scott Speicher, Daniel N. Miller, Lisa M. Durso, Xu Li, Bryan. L. Woodbury, Kent M. Eskridge, Amy Millmier Schmidt
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Abstract

Field application of beef cattle feedlot runoff may transport manure-borne microbes and antibiotic resistant bacteria to agricultural soils eventually impacting deeper soils and groundwater. To evaluate this potential, total soil, antibiotic resistance (AR), and fecal indicator bacteria (Escherichia coli and Enterococcus) and the presence/abundance of AR genes were examined to a depth of 1.8 m in an agricultural field receiving long-term application of feedlot runoff and compared to a nearby pasture receiving no runoff. While plate counts of total soil bacteria and cefotaxime-resistant, erythromycin-resistant, and tetracycline-resistant bacteria decreased with depth on both fields (p < 0.001) by an average 2-log10 colony forming unit g−1 to 1.8-m depth, field differences were only observed with greater abundances of total soil and erythromycin-resistant bacteria (p ≤ 0.026) in the runoff-amended versus control field soils. Soil bacterial and fecal indicator bacterial isolates evaluated phenotypically for resistance to 12 antibiotics varied in range and sensitivity. Using a culture-independent approach, erm(C) and tet(Q) were detected using polymerase chain reaction in 31% and 58% of runoff-field samples throughout the soil profile. Detection of erm(C) and tet(Q) in the control field soil profile was less frequent (0% and 11%, respectively). Two other genes, erm(A) and tet(X) were not detected in any soil samples. Based upon these results, long-term applications of beef cattle feedlot runoff may increase the total abundance of microorganisms in the surface and shallow soil, but the relative enrichment of AR was dependent upon the type of resistance evaluated and, more specifically, the genes targeted for analysis.

Abstract Image

肉牛饲养场径流对土壤抗菌素抗药性的影响
在田间施用肉牛饲养场径流可能会将粪便携带的微生物和抗生素耐药菌带到农田土壤中,最终影响深层土壤和地下水。为了评估这种可能性,我们对长期施用饲养场径流的农田中 1.8 米深的土壤细菌总数、抗生素耐药性 (AR) 细菌、粪便指示细菌(大肠杆菌和肠球菌)以及 AR 基因的存在/丰度进行了检测,并与附近未施用径流的牧场进行了比较。虽然两块田的土壤细菌总数和耐头孢他啶菌、耐红霉素菌和耐四环素菌的平板计数都随着深度的增加而减少(p <0.001),1.8 米深度的平均菌落形成单位为 2-log10 g-1,但只有在经径流改良的土壤与对照田土壤中观察到了更大的土壤细菌总数和耐红霉素菌的丰度差异(p ≤ 0.026)。土壤细菌和粪便指示细菌分离物对 12 种抗生素的表型耐药性评估范围和敏感性各不相同。通过聚合酶链式反应,在整个土壤剖面中,分别有 31% 和 58% 的径流田样本中检测到了erm(C) 和 tet(Q)。在对照田土壤剖面中,erm(C)和 tet(Q)的检测频率较低(分别为 0% 和 11%)。其他两个基因,erm(A) 和 tet(X) 没有在任何土壤样本中检测到。根据这些结果,长期施用肉牛饲养场径流可能会增加表层和浅层土壤中微生物的总丰度,但 AR 的相对富集取决于所评估的抗性类型,更具体地说,取决于分析所针对的基因。
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来源期刊
Agrosystems, Geosciences & Environment
Agrosystems, Geosciences & Environment Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
2.60
自引率
0.00%
发文量
80
审稿时长
24 weeks
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