Therapeutic efficacy of catechins on systemic complications of rheumatoid arthritis in animal model

Abstract

Green tea catechins are polyphenolic compounds that exhibit hallmark anti-inflammatory, antioxidant, and anti-arthritic properties especially epigallocatechin-3-gallate the major and the most abundant green tea catechin. The main goal of this study is to evaluate the role of catechins extracted from green tea on hematological abnormalities and impairments of both liver and kidney functions because of rheumatoid arthritis complications. In this study hot water extraction method was used for high-yield catechin separation, the crude extract was introduced to silica gel column chromatography for purifications, and the obtained catechin was detected by spectrophotometric assaying tests and FT-IR. Complete Freund’s adjuvant was used for in vivo arthritic induction. The results showed that green tea catechins enhanced anemic conditions associated with chronic diseases like rheumatoid arthritis, raised the erythrocyte count, and reduced leukocytosis. Catechins also improved the liver functions of rheumatoid arthritis rats as working by reducing the serum level of liver enzymatic biomarkers alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) from 51.37