Meta-Topolin Induced Shoot Organogenesis and Plant Regeneration from Different Explants of Tomato (Solanum lycopersicum L.)

Abstract

Tomato (Solanum lycopersicum L.) is the most prominent vegetable crop in the Solanaceae family. The varying concentrations of meta-Topolin (mT) evaluated at 2.0 mg/l generated the highest number of shoots than other purine-type cytokinins, like 6-Benzylaminopurine (BAP) and Kinetin (KIN) from diverse explants of tomato. The addition of Indole 3-acetic acid (IAA) at 0.1 mg/l with 2.0 mg/l mT triggered the production of the optimum number of shoots (18.6) from cotyledon than hypocotyl (13.8) and leaf (11.9) explants obtained from two genotypes of tomato cultivars. The ArkaSamrat (AS) cultivar was observed to be better responsive for induction and maximum production of shoots than the ArkaRakshak (AR) cultivar of tomato. The shoots were separated from the clusters of shoots and then cultured to the rooting medium containing IAA at 1.0 mg/l, which was found to be the most suitable concentration for induction rooting in both tomato cultivars. The complete plants were shifted to the greenhouse and recorded 93% and 89% survival rates in AS and AR cultivars, respectively. The regenerated plants did not show any variation in morphology with their mother plants. This plant regeneration system can be feasible for genetic improvement through the Agrobacterium-mediated transformation and generation of genome-targeted mutants using genome editing tools.