Molecular Characterization of Azotobacter and PSB from Xerophytic Plants of Maharashtra State, India

Abstract

The present study was conducted at Department of Plant Pathology and Agricultural Microbiology, MPKV, Rahuri during year 2021 to 2023 to characterize and screen different isolates of Azotobacter and PSB from Different Xerophytic plants from Different districts of Maharashtra State. 68 Azotobacter isolates were biochemically characterized and screened under In vitro conditions for their plant growth promoting properties. This isolates were biochemically characterized and screened In-vitro condition for their plant growth promoting properties. DNA polymorphism of isolates was studied using randomly amplified polymorphic DNA analysis. The genetic diversity among the bacterial strains was assessed by RAPD analysis. All the isolates showed reproducible DNA banding pattern. Diversity among the bacterial strains was assessed on the basis of variation of size number and intensity of bands.