Challenges and Strategies to develop RP-HPLC Method of L-Arginine with Polyphenolic compounds

Q3 Pharmacology, Toxicology and Pharmaceutics

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI:10.52711/0974-360x.2024.00018

Palwinder Kaur, Manish Vyas, Surajpal Verma

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引用次数: 0

Abstract

While developing a method of RP-HPLC for a new compound or combination of drugs simultaneously, many challenges are faced by the researcher pertaining to effective elution and sufficient resolution of a mixture of compounds. Many factors come into the picture, which reflects the ultimate elution of chemical compounds. Factors like the physicochemical properties of chemical compounds, the chemical nature of the mobile phase, instrumental factors, and experimental conditions play crucial roles in RP-HPLC method development. This research article discusses the challenges faced while developing a method of L-Arg along with polyphenolic compounds. Both the compounds have contrasting solubility profiles as L-Arg is soluble in water but PIC is soluble in organic solvents. Strategies were used to develop RP-HPLC of this combination with mobile phases like acetonitrile, orthophosphoric acid, methanol, and potassium dihydrogen phosphate buffer of pH 2.6. The present article provides an insightful approach to develop a new RP-HPLC method for a combination of compounds having related physicochemical characteristics.

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开发左旋精氨酸与多酚类化合物的 RP-HPLC 方法的挑战与策略

在为一种新化合物或药物组合同时开发 RP-HPLC 方法时，研究人员面临着许多挑战，这些挑战涉及化合物混合物的有效洗脱和充分分辨。许多因素都会影响化合物的最终洗脱。化合物的理化性质、流动相的化学性质、仪器因素和实验条件等因素在 RP-HPLC 方法开发中起着至关重要的作用。本研究文章讨论了在开发 L-Arg 和多酚化合物的方法时所面临的挑战。这两种化合物的溶解度截然不同，L-Arg 易溶于水，而 PIC 易溶于有机溶剂。研究人员利用乙腈、正磷酸、甲醇和 pH 值为 2.6 的磷酸二氢钾缓冲液等流动相，开发了这种组合的 RP-HPLC 方法。本文为具有相关理化特性的化合物组合的 RP-HPLC 新方法的开发提供了一种有见地的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research Journal of Pharmacy and Technology Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal, devoted to pharmaceutical sciences. The aim of RJPT is to increase the impact of pharmaceutical research both in academia and industry, with strong emphasis on quality and originality. RJPT publishes Original Research Articles, Short Communications, Review Articles in all areas of pharmaceutical sciences from the discovery of a drug up to clinical evaluation. Topics covered are: Pharmaceutics and Pharmacokinetics; Pharmaceutical chemistry including medicinal and analytical chemistry; Pharmacognosy including herbal products standardization and Phytochemistry; Pharmacology: Allied sciences including drug regulatory affairs, Pharmaceutical Marketing, Pharmaceutical Microbiology, Pharmaceutical biochemistry, Pharmaceutical Education and Hospital Pharmacy.