Assessment and Mitigation of Bacterial and Fungal Contamination in Refrigerator Waterlines

Paul Dawson, Julie Northcutt, A. Buyukyavuz, Belinda Cochran, Thomas McCollough
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Abstract

Three experiments were conducted on the recovery of microorganisms associated with refrigerator water lines. In the first experiment, between 2 and 3 logs CFU/ml were recovered from 100 different refrigerators’ residential water and ice lines. In a second experiment, plastic tubing segments cut from commercial refrigerator waterline systems were inoculated with the bacterium Pseudomonas aeruginosa and a fungal strain of Aspergillus flavus. Either 0.02% peracetic acid (PAA) exposure for 2 min or 0.03% PAA exposure for 1 min resulted in no recoverable bacteria cells, however, 0.25% PAA contact for 2 min was needed to eliminate detection of fungal cells. The third experiment tested sanitation of a water system inoculated with a mixed culture of P. aeruginosa and A flavus with a water filter cartridge filled with 3.5% PAA. The 3.5% concentration was determined using a computer simulation for mixing during a cleaning cycle that would yield a minimum concentration of at least 0.25% throughout the water system. After loading a water filter cartridge containing 3.5% PAA, allowing water to flow into the system for 15 s, and then allowing the system to sit for 5 min, no viable P. aeruginosa or A flavus cells were recovered up to 10 days after treatment.
评估和减轻冰箱水管中的细菌和真菌污染
对冰箱水管相关微生物的回收进行了三次实验。在第一项实验中,从 100 台不同冰箱的家用水管和冰管中回收了 2 至 3 logs CFU/ml。在第二个实验中,从商用冰箱水管系统中切下的塑料管段接种了铜绿假单胞菌和黄曲霉的真菌菌株。将 0.02% 的过乙酸 (PAA) 接触 2 分钟或将 0.03% 的过乙酸接触 1 分钟后,都无法回收细菌细胞,但需要将 0.25% 的过乙酸接触 2 分钟才能检测出真菌细胞。第三个实验测试了用装有 3.5% PAA 的滤水器滤芯对接种了铜绿假单胞菌和黄曲霉菌混合培养物的水系统进行消毒。3.5% 的浓度是通过计算机模拟确定的,以便在清洗周期中进行混合,使整个水系统中的最低浓度至少达到 0.25%。在装入含有 3.5% PAA 的滤水器滤芯后,让水流进系统 15 秒钟,然后让系统静置 5 分钟,处理后 10 天内未发现有存活的铜绿微囊藻或黄曲霉菌细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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