The co-inoculation effect of Azotobacter spp. and Trichoderma asperellum with inorganic fertilizer on growth and yield of carrot

T.T. Gunasekara, K. Nawarathna, A. Aruggoda, S. Kohombange
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Abstract

- Fertilizers play a prominent role in increasing the productivity of crops in the country. Phosphate solubilizing and nitrogen fixing microorganisms based biofertilizers can be used to reduce the inorganic nitrogen and phosphorous based chemical fertilizer usage and increase the plant availability nitrogen and phosphorous requirement. This study was aimed to use Sri Lankan isolates Azotobacter spp. and Trichoderma asperellum combination with lower rates of chemical fertilizers to evaluate their efficiency as biofertilizers for growth and yield characters of carrot. Further, possibility of combination use of both microorganisms were assessed. The pot experiment was carried out in Completely Randomized Design (CRD), consisting six treatments and four replications. The Treatments were namely T1 (No Fertilizer), T2 (100% NPK - DOA Recommendation), T3 (50% NP + K), T4 (50% NP + Azotobacter spp. + Trichoderma asperellum + K), T5 (75% NP +K) and T6 (75% NP + Azotobacter spp. + Trichoderma asperellum + K). The results of compatibility test resulted with 100% compatibility of Azotobacter spp. and Trichoderma asperellum isolates respectively. According to the results of pot experiment, best results of growth and yield parameters were observed from treatments T4, T6 and T2. The results of the present study reveals that combined use of Trichoderma asperellum and Azotobacter spp. with reduced levels of (25% and 50%) nitrogen and phosphorous based inorganic fertilizers could enhance the growth and yield of carrot equivalent or higher than those obtained by using full rates of Department of Agriculture Sri Lanka recommended inorganic fertilizers.
氮杂菌和赤霉菌与无机肥联合接种对胡萝卜生长和产量的影响
- 肥料在提高本国农作物产量方面发挥着重要作用。以磷酸盐溶解和固氮微生物为基础的生物肥料可用于减少无机氮和磷化学肥料的使用量,提高植物对氮和磷的需求量。这项研究的目的是将斯里兰卡分离的氮杆菌和毛霉与较低比例的化肥结合使用,以评估它们作为生物肥料对胡萝卜生长和产量特征的影响。此外,还评估了两种微生物联合使用的可能性。盆栽试验采用完全随机设计(CRD),包括六个处理和四个重复。处理分别为 T1(不施肥)、T2(100% 氮磷钾 - DOA 建议)、T3(50% 氮磷钾)、T4(50% 氮磷钾 + 根瘤菌 + 根瘤菌)、T5(75% 氮磷钾)和 T6(75% 氮磷钾 + 根瘤菌 + 根瘤菌 + 根瘤菌)。相容性测试结果表明,根瘤菌和毛癣菌的相容性分别为 100%。根据盆栽试验的结果,T4、T6 和 T2 处理的生长和产量参数结果最好。本研究结果表明,在减少氮肥和磷肥用量(25% 和 50%)的情况下,联合使用灰霉和氮杂菌,可以提高胡萝卜的生长和产量,其效果相当于或高于斯里兰卡农业部推荐的全量无机肥料。
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