Evaluation of human mononuclear umbilical cord blood cells systemic administration efficiency in the acute period of experimental severe spinal cord injury

V. Smirnov, S. I. Ryabov, M. Zvyagintseva, S. Bazanovich, Ya. V. Morozova, S. M. Radaev, A. E. Talypov, A. Grin
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Abstract

Aim. To evaluate the efficiency of systemic (intravenous) application of cryopreserved human umbilical cord blood mononuclear cells (HUCBCs) in animal models of acute contusion spinal cord injury for the restoration of hind limb motor function and formation of posttraumatic cysts using clinically significant examination methods.Materials and methods. Adult female Sprague–Dowley rats were used for the study. Severe acute contusion spinal cord injury model was performed using standard “weight‑drop” method. All samples of cryopreserved HUCBCs concentrate were prestored prior to infusion for 3 to 4 years at –196 °C. Hind limbs motor function was evaluated using open‑field technique and standard BBB testing system. Magnetic resonance scanning was performed using high‑field magnetic resonance CleanScan 7.0 T tomography (Bruker BioSpin, Germany).Results. Intravenous infusions of HUCBCs were performed on Day 1 following acute severe spinal cord injury. Motor function assessment demonstrated significant (p <0.05) improvement of hind limbs motor function (up to 40–50 %) comparing to self‑healing outcomes. Moreover, by the Days 4 and 5 after severe spinal cord injury, the volume of posttraumatic cystic cavity decreases significantly (up to 40 %) (p <0.05).Conclusion. The obtained results demonstrated that cryopreserved HUCBCs can be used as an effective source for cell therapy of acute contusion spinal cord injury.
评估人单核脐带血细胞在实验性严重脊髓损伤急性期的全身给药效率
目的评估在急性挫伤脊髓损伤动物模型中全身(静脉)应用低温保存的人脐带血单核细胞(HUCBCs)对恢复后肢运动功能和形成创伤后囊肿的效率,采用具有临床意义的检查方法。研究使用成年雌性 Sprague-Dowley 大鼠。严重急性挫伤脊髓损伤模型采用标准的 "体重下降 "法。所有冷冻保存的 HUCBCs 浓缩物样本在输注前均在 -196 °C 下预存 3 至 4 年。采用开场技术和标准 BBB 测试系统对后肢运动功能进行评估。使用高场磁共振CleanScan 7.0 T断层扫描(德国布鲁克BioSpin公司)进行磁共振扫描。急性严重脊髓损伤后第 1 天进行了 HUCBCs 静脉注射。运动功能评估结果表明,与自愈结果相比,后肢运动功能有了明显改善(p <0.05)(达 40-50%)。此外,在严重脊髓损伤后的第4天和第5天,创伤后囊腔的体积明显缩小(达40%)(P <0.05)。研究结果表明,冷冻保存的 HUCBCs 可作为急性挫伤性脊髓损伤细胞治疗的有效来源。
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