Solid state fermentation characteristic of rice straw using herbivore s cecum microbes

Abstract

This study was aimed at identifying highly capable lignolytic microbes from nature for use in Solid State Fermentation (SSF) of rice straw. The SSF silage was prepared in laboratory scale, as the following treatments: uninoculated (control), Lactobacillus plantarum FCC 123 (LP), fiber-degrading fungi (Aspergillus sp.) from horse cecum (FF), and fiber-degrading bacteria (Enterococcus casseliflavus) from buffalo cecum (FB). Incubation was carried out for a month at room temperature. The observed parameters were: organic acids, water-soluble carbohydrate (WSC), microorganism and nutrient composition. Rice straw SSF that was inoculated with LP showed the highest quality of fermentation, indicated by significant highest lactic acid bacteria (LAB) population, and has the lowest of poor bacteria indicators (coliform, aerobic bacteria, and bacilli). The LP treatment also has the highest LAB content and lowest WSC. Among treatments, FB treatment seems to have given a similar result with LP followed by FF. While the chemical composition seems unaffected by treatments. Compared with the fresh material, all fermentation with and without inoculants has reduced neutral detergent fiber (NDF) and increased acid detergent fiber (ADF), but there were no differences among all treatments. Inoculation of both LP and FB could improve rice straw SSF silage quality, but this system could not improve fiber degradation as well as in liquid state fermentation (LSF). Keywords: Microorganism, Organic acid, Silage, Straw, Water soluble carbohydrate