Detection of Biosynthesis of CuNPs using P. aeruginosa Isolated from Soil

Abstract

- The production of nanoparticles, while controlling the size and shape of the particles, has recently occupied much research in the field of nanotechnology. The biosynthesis of metal nanoparticles is of great importance due to their suitability for the environment. The use of microorganisms to produce nanoparticles has progressed over chemical and physical methods due to their effectiveness, low cost, and environmentally friendly nature. The present study focused on the synthesis of copper nanoparticles (CuNPs) in a simple, efficient, and rapid manner by the bacterium Pseudomonas aeruginosa. Copper is important in this process due to its interesting physical and chemical properties. It has good therapeutic potential, and when copper-sulphate-solution is added to the bacterial suspension devoid of bacterial cells, it is observed that the color of the reaction mixture changes to green, and this is evidence of the formation of nanoparticles. Different techniques were relied upon to characterize CuNPs by ultraviolet spectroscopy, with the highest value at 314. An FT-IR examination was conducted to determine the active aggregates. A SEM examination was also conducted, confirming that the size of copper nanoparticles reached 56.40 nm. This study aimed to produce nanoparticles. CuNPs using a Pseudomonas aeruginosa.