Effect of Giving Black Seed (Nigella Sativa L) On Il-10 Levels, And CRP Levels (Experimental Study on Wistar Strain Male White Rats Induced By A High Fat Diet)

Abstract

Dyslipidemia is a disease that often occurs in Indonesia. The impacts of this disease include inflammation and various types of cytokines. This study aimed to determine the effect of Black Seed on Interleukin 10 levels and CRP levels in rats induced by a high-fat diet. This research is a posttest-only control group type of research. Twenty-five male white Wistar rats were taken as samples and divided into 5 groups: K0 (pellets and water), K – (high-fat diet), K + (high-fat diet + simvastatin 1.8 mg/day), P1 (high-fat diet + Black Seed 3 ml/KgBB), P2 (high-fat diet + black seed 4 ml/KgBB). The data required in this study was obtained through the ELISA test. The collected data was analyzed using one-way ANOVA and post hoc LSD test. From the results of the analysis carried out, it was found that (1) the difference in mean IL-10 levels between groups was significant with a p-value <0.05; (2) The difference in average CRP levels between groups where K0, K-, and P1 is significant, while between K (+) and P2 is not significant with a p>0.05. Administration of Black Seed affected IL-10 levels and CRP levels in white mice induced by a high-fat diet.