Study of Staphylococcus aureus genotyping using Coagulase gene method

Q4 Earth and Planetary Sciences

Research Journal of Chemistry and Environment Pub Date : 2024-01-31 DOI:10.25303/283rjce040047

Sadah Al-Halfi Alaa S., Al-Shammari Maher Zaki Faisal, Khalil Ibrahim Suaad

引用次数: 0

Abstract

Fifty isolates of Staphylococcus aureus bacteria were obtained out of 200 samples collected from clinical sources. The highest resistance to S. aureus bacteria was 100% Benzylpericillin, Oxacillin and Moxiflaxocin antibiotics, 86% Levoflaxacin resistance, 76% Erythromycin resistance, 66% Tetracycline resistance, 64% Tobramycin resistance, 62% Gentamicin resistance, 60% Clindamycin resistance and 58% Trimethoprim/Sulfamethoxazole resistance. It was 38% sensitive to Gentamicin and Clindamycin, 36% sensitive to Tobramycin, 22% sensitive to Erythromycin and 14% sensitive to Levoflaxacin while the results showed the presence of five different sized types of DNA packets when detecting Coa gene of S.aureus bacteria using PCR techniques.

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使用凝固酶基因法对金黄色葡萄球菌进行基因分型的研究

从临床采集的 200 个样本中分离出 50 个金黄色葡萄球菌。金黄色葡萄球菌对苄星青霉素、氧西林和莫西沙星抗生素的耐药性最高，分别为 100%、86%、76%、76%、66%、64%、62%、62%、60% 和 58%，其中对苄星青霉素、氧西林和莫西沙星抗生素的耐药性最高，对左氧氟沙星的耐药性最高，对红霉素的耐药性最高，对四环素的耐药性最高，对妥布霉素的耐药性最高，对庆大霉素的耐药性最高，对克林霉素的耐药性最高，对三甲双胍/磺胺甲噁唑的耐药性最高。利用 PCR 技术检测金黄色葡萄球菌的 Coa 基因时，结果显示存在五种不同大小的 DNA 包。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊