Mimosine concentration in giant leucaena (Leucaena leucocephala subsp. glabrata) fluctuates with age and plant part

IF 0.9 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Tropical Grasslands-Forrajes Tropicales Pub Date : 2024-01-31 DOI:10.17138/tgft(12)11-23

M. Honda, D. Borthakur

{"title":"Mimosine concentration in giant leucaena (Leucaena leucocephala subsp. glabrata) fluctuates with age and plant part","authors":"M. Honda, D. Borthakur","doi":"10.17138/tgft(12)11-23","DOIUrl":null,"url":null,"abstract":"Giant leucaena is a multipurpose tree-legume found throughout the tropics and sub-tropics. Its foliage is used as animal fodder due to high protein and fiber. Giant leucaena has several other advantageous traits such as fast-growth, high yields and strong tolerance to environmental stresses. Despite having these desirable qualities, leucaena tissues contain an undesirable attribute, a toxic non-protein amino acid, mimosine, found in all parts of the plant including the foliage. The goal of this research was to determine mimosine concentrations in various tissues and life stages of giant leucaena plants to inform use of leucaena foliage as a fodder. Mimosine was extracted from different parts of giant leucaena at different ages and quantified using HPLC analysis. qRT-PCR was used to determine the relative expression of mimosine synthase in leucaena tissues. Mimosine was present in all parts of the leaf, stem and root of giant leucaena, and concentrations changed depending on the age of the plant. Green seeds had the highest expression level of mimosine synthase. Mimosine is ubiquitous and abundant in leucaena tissues with younger and immature plants and tissues containing more mimosine than older mature plants and tissues.","PeriodicalId":56049,"journal":{"name":"Tropical Grasslands-Forrajes Tropicales","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Grasslands-Forrajes Tropicales","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.17138/tgft(12)11-23","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Giant leucaena is a multipurpose tree-legume found throughout the tropics and sub-tropics. Its foliage is used as animal fodder due to high protein and fiber. Giant leucaena has several other advantageous traits such as fast-growth, high yields and strong tolerance to environmental stresses. Despite having these desirable qualities, leucaena tissues contain an undesirable attribute, a toxic non-protein amino acid, mimosine, found in all parts of the plant including the foliage. The goal of this research was to determine mimosine concentrations in various tissues and life stages of giant leucaena plants to inform use of leucaena foliage as a fodder. Mimosine was extracted from different parts of giant leucaena at different ages and quantified using HPLC analysis. qRT-PCR was used to determine the relative expression of mimosine synthase in leucaena tissues. Mimosine was present in all parts of the leaf, stem and root of giant leucaena, and concentrations changed depending on the age of the plant. Green seeds had the highest expression level of mimosine synthase. Mimosine is ubiquitous and abundant in leucaena tissues with younger and immature plants and tissues containing more mimosine than older mature plants and tissues.

查看原文本刊更多论文

大叶女贞（Leucaena leucocephala subsp.glabrata）中的亚胺嗪浓度随树龄和植株部位而波动

大叶女贞是一种多用途树状豆科植物，在整个热带和亚热带地区都有分布。它的叶子具有高蛋白和高纤维，可用作动物饲料。大叶女贞还具有其他一些有利特性，如生长快、产量高和对环境压力的耐受力强。尽管具有这些理想特性，但白芒花组织中含有一种不良属性，即一种有毒的非蛋白氨基酸--含羞草碱，它存在于植物的各个部分，包括叶片。这项研究的目的是确定巨型白芒花植物不同组织和生命阶段中的含羞草碱浓度，为将白芒花叶片用作饲料提供参考。研究人员从不同年龄段的大叶白头翁不同部位提取了含羞草碱，并使用高效液相色谱法对其进行了定量分析，同时使用 qRT-PCR 技术确定了白头翁组织中含羞草碱合成酶的相对表达量。大叶女贞的叶、茎和根的所有部位都含有亚胺嘧啶，其浓度随植株年龄的变化而变化。绿色种子的含羞草碱合成酶表达水平最高。含羞草苷在白花蛇舌草组织中无处不在且含量丰富，幼苗和未成熟植株及组织的含羞草苷含量高于较老的成熟植株和组织。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Tropical Grasslands-Forrajes Tropicales Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

16 weeks

期刊介绍： The Journal publishes, in English or Spanish, Research Papers and Short Communications on research and development, as well as contributions from practitioners (Farmer Contributions) and Review Articles, related to pastures and forages in the tropics and subtropics. There is no regional focus; the information published should be of interest to a wide readership, encomprising researchers, academics, students, technicians, development workers and farmers. In general, the focus of the Journal is more on sown (''improved'') pastures and forages than on rangeland-specific aspects of natural grasslands, but exceptions are possible (e.g. when a submission is relevant for a particularly broad readership in the pasture and forage science community). The Journal will also consider the occasional publication of associated, but closely related, research in the form of an additional scientific communication platform [e.g. a re-make of the former Genetic Resources Communication series of the former Division of Tropical Crops and Pastures of the Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia]. Areas of particular interest to the Journal are: Forage Genetic Resources and Livestock Production[...] Environmental Functions of Forages[...] Socio-economic Aspects[...] Topics within the aforementioned areas may include: Diversity evaluation; Agronomy; Establishment (including fertilization); Management and utilization; Animal production; Nutritive value; Biotic stresses (pests and diseases, weeds); Abiotic stresses (soil fertility, water, temperature); Genetics and breeding; Biogeography and germplasm collections; Seed production; Ecology; Physiology; Rhizobiology (including BNF, BNI, mycorrhizae); Forage conservation; Economics; Multilocational experimentation; Modelling.