Phytochemistry, Mineral Estimation, Nutritional, and the In Vitro Anti-Sickling Potentials of Oil Extracted from the Seeds of Mucuna Flagellipes

IF 1.1 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Journal of the Mexican Chemical Society Pub Date : 2024-02-01 DOI:10.29356/jmcs.v68i2.1898

Emmanuel U. Ejiofor, Alwell C. Ako, Maxwell T. Kube, E. Agwamba, Chinweuba Alala, Kelvin Maduabuchi, M. Ejiofor

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Abstract

Abstract. Sickle cell disease is an inherited blood disorder indicative of red blood cells becoming sickle-shaped. The study investigated the in vitro anti-sickling potentials of the seed oil of M. flagellipes. The phytochemistry (fatty acids, vitamins, and minerals) was also determined using standard protocols. Finally, nutritional calculations on the oil were performed to determine its suitability for nutritional purposes. The result showed high zinc content (780 ± 2.50 µg/ 100 mg), while low iron content was observed (170 ± 1.30 µg/ 100 mg). Vitamin analysis showed the presence of vitamins A, E and K with values of 220 ± 1.60, 370 ± 2.20, and 197 ± 0.23 µg/100 mg respectively. The fatty acid profile revealed oleic (31.87 %) and linoleic (18.30 %) fatty acids as the major unsaturated fat in the oil, while palmitic fatty acid (5.91 %) was the major saturated fatty acid. Nutritional calculations showed high PUFA/SFA (2.07), MUFA/SFA (3.62), and UI (68.47). However, the index of thrombogenicity (0.07) and atherogenicity (0.11) was low in the seed oil. Finally, the in vitro anti-sickling potentials of the seed oil showed the oil inhibited and reversed sickling in a dose-dependent manner. Hbs polymerization was also inhibited and Fe2+/Fe3+ was upregulated following treatment with the seed oil. Collectively, the oil showed good anti-sickling potentials, which can be labelled to the presence of zinc, vitamins and unsaturated fat. The nutritional calculations suggest that the seed oil is cardio-friendly and does not pose any nutritional threat.

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从旗叶木瓜种子中萃取的油的植物化学、矿物质估算、营养和体外抗酸痛潜力

摘要镰状细胞病是一种遗传性血液疾病，表现为红细胞变成镰状。本研究调查了鞭毛菜种子油的体外抗镰状细胞病潜力。研究还采用标准方案测定了植物化学成分（脂肪酸、维生素和矿物质）。最后，还对种子油进行了营养计算，以确定其是否适合用于营养目的。结果显示，锌含量较高（780 ± 2.50 µg/100 mg），而铁含量较低（170 ± 1.30 µg/100 mg）。维生素分析表明存在维生素 A、E 和 K，含量分别为 220 ± 1.60、370 ± 2.20 和 197 ± 0.23 微克/100 毫克。脂肪酸谱显示，油中的主要不饱和脂肪是油酸（31.87%）和亚油酸（18.30%），而饱和脂肪酸是棕榈酸（5.91%）。营养计算显示，PUFA/SFA（2.07）、MUFA/SFA（3.62）和 UI（68.47）均较高。不过，种子油的血栓形成指数（0.07）和动脉粥样硬化指数（0.11）较低。最后，种子油的体外抗镰状细胞潜力表明，种子油能以剂量依赖的方式抑制和逆转镰状细胞。经种子油处理后，Hbs 聚合也受到抑制，Fe2+/Fe3+ 上调。总之，种子油显示出良好的抗镰刀形病变潜力，这可归因于锌、维生素和不饱和脂肪的存在。营养计算表明，种子油对心血管有益，不会造成任何营养威胁。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Mexican Chemical Society 化学-化学综合

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of the Mexican Chemical Society (J. Mex. Chem. Soc.) is a scientific, blind, peer reviewed, and open access, free of charge publication that covers all areas of chemistry and its sub-disciplines (i.e. medicinal chemistry, natural products, electrochemistry, material science, computational chemistry, organic chemistry, bionirganic chemistry, etc). It is devoted to facilitating the worldwide advancement of our understanding of chemistry. It will primarily publish original contributions of research in all branches of the theory and practice of chemistry in its broadest context as well as critical reviews in active areas of chemical research where the author has published significant contribution. The J. Mex. Chem. Soc. is a quarterly publication which language of submission and publication is English. To be suitable for publication in J. Mex. Chem. Soc., manuscripts must describe novel aspects of chemistry, high quality of results and discussion an excellent bibliographic support, and contribute to the development of the field. Routine or incremental work are not suitable for publication in J. Mex. Chem. Soc. Authors are encouraged to send contributions in electronic form. Our online submission system guides you stepwise through the process of entering your article details and uploading your files.