Obtaining Edible Pullulan-based Films with Antimicrobial Properties

IF 0.5 Q4 CHEMISTRY, MULTIDISCIPLINARY
L.V. Ignatova, Z. K. Urazova, Y. Brazhnikova, N.V. Vedyashkina
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引用次数: 0

Abstract

A nutrient medium was selected for the efficient production of exopolysaccharide (EPS) by A. pullulans C7 strain. The production of pullulan polysaccharide was evaluated on nutrient media with traditional carbon sources and cheap substrates that were plant wastes. For maximum EPS accumulation, we proposed an optimized Czapek-Dox medium with glucose as a carbon source, sodium nitrate as a nitrogen source, and C/N=232:1 ratio (EPS yield 12.79±0.64 g/l). Medium with grape pomace 5% (EPS yield was 15.08±0.34 g/l) and medium with topinambour tuber hydrolysate 5% (EPS yield was 14.44±0.21 g/l) was proposed as a cheap substrate. Edible films with antimicrobial activity were obtained on the basis of the isolated polysaccharide. The antibacterial activity of films against Escherichia coli 603 and Staphylococcus aureus ST228 was shown when essential oils of rosemary (zones of growth inhibition from 8.41±0.71 to 9.98±0.32 mm) and oregano (zones of growth inhibition from 8.09±0.51 to 9.54±0.24 mm) were added to pullulan. The addition of xanthan gum and glycerol to the films increased their strength and elasticity. The infrared spectrum of the pullulan film showed absorption bands characteristic of polysaccharide structures.
获得具有抗菌特性的可食用普鲁兰基薄膜
研究人员选择了一种营养培养基,以利于 A. pullulans C7 菌株高效生产外多糖(EPS)。在含有传统碳源和廉价植物废料底物的营养培养基上,对拉氏菌多糖的生产进行了评估。为了最大限度地积累 EPS,我们提出了一种优化的 Czapek-Dox 培养基,以葡萄糖为碳源,硝酸钠为氮源,C/N=232:1(EPS 产量为 12.79±0.64 克/升)。建议使用含 5%葡萄渣的培养基(EPS 产量为 15.08±0.34 克/升)和含 5%块茎水解物的培养基(EPS 产量为 14.44±0.21 克/升)作为廉价基质。在分离多糖的基础上,获得了具有抗菌活性的食用薄膜。当迷迭香精油(生长抑制区为 8.41±0.71 至 9.98±0.32 毫米)和牛至精油(生长抑制区为 8.09±0.51 至 9.54±0.24 毫米)加入到拉伸聚糖中时,薄膜对大肠杆菌 603 和金黄色葡萄球菌 ST228 具有抗菌活性。在薄膜中添加黄原胶和甘油可增加薄膜的强度和弹性。拉鲁兰薄膜的红外光谱显示出多糖结构所特有的吸收带。
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来源期刊
Eurasian Chemico-Technological Journal
Eurasian Chemico-Technological Journal CHEMISTRY, MULTIDISCIPLINARY-
CiteScore
1.10
自引率
20.00%
发文量
6
审稿时长
20 weeks
期刊介绍: The journal is designed for publication of experimental and theoretical investigation results in the field of chemistry and chemical technology. Among priority fields that emphasized by chemical science are as follows: advanced materials and chemical technologies, current issues of organic synthesis and chemistry of natural compounds, physical chemistry, chemical physics, electro-photo-radiative-plasma chemistry, colloids, nanotechnologies, catalysis and surface-active materials, polymers, biochemistry.
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