Verification of a test system for detecting antibodies to bovine diarrhea virus using the enzyme immunoassay method “Cattle VD - SEROTEST plus”

Abstract

Relevance. Agricultural enterprises of the Russian Federation with intensive livestock farming are annually faced with the problem of a respiratory-intestinal nature, especially in young animals. This entails large losses. As a result of problems arising in animals, viral diarrhea of cattle occupies one of the leading places. The urgency of the problem lies in the large economic damage that occurs due to the birth of non-viable offspring, the death of newborn young animals, the development of various forms of pneumonia, decreased productivity, dysfunction of animal reproduction, as well as the costs of carrying out preventive, quarantine and liquidation measures. Reliable laboratory diagnostics allows you to correctly build a strategy to combat the disease. The article presents the results of verification of a test system for detecting antibodies to bovine diarrhea virus using the enzyme immunoassay method “Cattle VD — SEROTEST plus”.Methods. The diagnostic significance of the test system was assessed according to the indicators of sensitivity, specificity and precision under conditions of repeatability and reproducibility.Results. The coefficient of variation (CV) for the enzyme-linked immunosorbent assay ranged from 1.9 to 11.2%, indicating good consistency of results with intra-laboratory precision under conditions of repeatability and reproducibility.