Influence of ultrafine particles of copper and iron on the intestinal microbiocenosis of broiler chicken

Abstract

Relevance. Studying the bacterial diversity of the intestine under the influence of various factors makes it possible to study changes in the qualitative and quantitative composition under the influence of various factors.Methods. In our work, we studied the bacterial profile of samples of the contents of the cecum of broiler chickens. To assess the effect of ultrafine particles of Cu and Fe on the metabolism in the body of animals fed a semi-synthetic diet, 120 heads of one-week-old broiler chickens were selected, which were divided into 4 groups (n = 30) using the analog pair method. During the experiment, all birds were under the same housing and feeding conditions. The duration of the experiment was 28 days. To solve the problems, broiler chickens were used, which were supplemented with ultrafine Cu and Fe particles in their diet in order to study their effect on the microbiocenosis of the poultry digestive system. Using the analogue pair method, four groups of 30 broiler chickens each at the age of 7 days were formed. During the study, housing and feeding conditions were identical for all groups. The study was conducted over 4 weeks. For feeding, we prepared a semi-synthetic diet (K1) and a semi-synthetic diet, deficient in microelements, modified by us (K2).Results. The use of ultrafine copper and iron particles has been shown to alter the intestinal bacterial community of broiler chickens. Thus, based on the data, we can conclude that obligate bacteria in the cecum of birds can modulate the level of accumulation of chemical elements in the bird’s body when various substances are introduced. According to the data obtained, ultrafine iron particles made it possible to maintain the normal diversity of the digestive microbiota of poultry, which was expressed, among other things, in a decrease in the number of representatives of the Enterobacteriaceae family, which includes representatives of pathogenic and opportunistic taxa, while the introduction of ultrafine copper particles contributed to an increase in the number of representatives of this family.