Severity of COVID-19 Infectious and Immune-Based Profile

Pub Date : 2024-02-21 DOI:10.5812/jjm-143256
H. Shahi, Fatemeh Kiaee, Majid Marjani, E. Mortaz
{"title":"Severity of COVID-19 Infectious and Immune-Based Profile","authors":"H. Shahi, Fatemeh Kiaee, Majid Marjani, E. Mortaz","doi":"10.5812/jjm-143256","DOIUrl":null,"url":null,"abstract":"Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes immune system dysregulation and a systemic cytokine storm. Under healthy conditions, T helper cells protect against intracellular pathogens, extracellular parasites, and extracellular bacteria. Objectives: For the novelty of our study, little is known regarding the balance of T cell subtypes and responses in two forms of COVID-19 in our country. We investigated whether there was a relationship between T cell subtype frequency and cytokines by COVID-19 severity. Methods: Forty-six PCR-confirmed severe (n = 30) and moderate (n = 16) COVID-19 patients and 13 sex- and age-matched healthy control (HC) subjects were enrolled. Immunophenotyping of T cell subsets and related serum cytokines was performed using flow cytometry and ELISA, respectively. Results: There was a significantly lower frequency of CD8+Tbet+ (P < 0.01) T cells in the severe group compared to HC. Also, there was a significantly lower frequency of CD4+GATA3+ (P < 0.001) and CD8+Tbet+ (P < 0.001) T cells in the severe group compared to the moderate group. Moreover, receiver-operating characteristic (ROC) curve analysis revealed a considerable correlation between CTL (CD8+T-bet+) subtypes and the severity of the disease. Severe COVID-19 disease was associated with reduced interferon-gamma (IFN-γ) and interleukin (IL)-2 concentration and increased IL-5 and IL-6 concentration. Conclusions: Reduced systemic levels of IL-2 can trigger decreased numbers of Th1 and Th2 cells, and in contrast to elevated IL-5 and IL-6, the numbers of Th2 cells did not increase in these cases.","PeriodicalId":0,"journal":{"name":"","volume":"185 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.5812/jjm-143256","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes immune system dysregulation and a systemic cytokine storm. Under healthy conditions, T helper cells protect against intracellular pathogens, extracellular parasites, and extracellular bacteria. Objectives: For the novelty of our study, little is known regarding the balance of T cell subtypes and responses in two forms of COVID-19 in our country. We investigated whether there was a relationship between T cell subtype frequency and cytokines by COVID-19 severity. Methods: Forty-six PCR-confirmed severe (n = 30) and moderate (n = 16) COVID-19 patients and 13 sex- and age-matched healthy control (HC) subjects were enrolled. Immunophenotyping of T cell subsets and related serum cytokines was performed using flow cytometry and ELISA, respectively. Results: There was a significantly lower frequency of CD8+Tbet+ (P < 0.01) T cells in the severe group compared to HC. Also, there was a significantly lower frequency of CD4+GATA3+ (P < 0.001) and CD8+Tbet+ (P < 0.001) T cells in the severe group compared to the moderate group. Moreover, receiver-operating characteristic (ROC) curve analysis revealed a considerable correlation between CTL (CD8+T-bet+) subtypes and the severity of the disease. Severe COVID-19 disease was associated with reduced interferon-gamma (IFN-γ) and interleukin (IL)-2 concentration and increased IL-5 and IL-6 concentration. Conclusions: Reduced systemic levels of IL-2 can trigger decreased numbers of Th1 and Th2 cells, and in contrast to elevated IL-5 and IL-6, the numbers of Th2 cells did not increase in these cases.
分享
查看原文
COVID-19 感染和免疫特征的严重程度
背景:严重急性呼吸系统综合征冠状病毒 2(SARS-CoV-2)会导致免疫系统失调和全身细胞因子风暴。在健康状态下,T 辅助细胞能抵御细胞内病原体、细胞外寄生虫和细胞外细菌。研究目的我们的研究很新颖,在我国,人们对两种形式的 COVID-19 中 T 细胞亚型的平衡和反应知之甚少。我们根据 COVID-19 的严重程度调查了 T 细胞亚型频率与细胞因子之间是否存在关系。研究方法纳入 46 名经 PCR 确认的重度(n = 30)和中度(n = 16)COVID-19 患者以及 13 名性别和年龄匹配的健康对照(HC)受试者。分别使用流式细胞术和酶联免疫吸附法对 T 细胞亚群和相关血清细胞因子进行免疫分型。结果显示与 HC 相比,重症组的 CD8+Tbet+ T 细胞频率明显较低(P < 0.01)。此外,与中度组相比,重度组 CD4+GATA3+ (P < 0.001) 和 CD8+Tbet+ (P < 0.001) T 细胞的频率也明显较低。此外,接收器操作特征(ROC)曲线分析显示,CTL(CD8+T-bet+)亚型与疾病严重程度之间存在相当大的相关性。严重的 COVID-19 疾病与γ干扰素(IFN-γ)和白细胞介素(IL)-2 浓度降低以及 IL-5 和 IL-6 浓度升高有关。结论是全身IL-2水平降低会导致Th1和Th2细胞数量减少,与IL-5和IL-6升高相反,Th2细胞数量在这些病例中并未增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信