Role of Granulocyte Colony Stimulating Factor Effects on Unresponsive Thin Endometrium in Women Undergoing Controlled Ovarian Stimulated Intrauterine Insemination Cycles

Abstract

Back Ground: It is a prospective observational study that included unexplained infertility cycles with COS –IUI protocols. Thin endometrium of less than 7mm has a negative effect on pregnancy rate. Endometrial function and receptivity factors are important in the success of implantation. Aim: To monitor the effects of G-CSF on thin endometrium in improving endometrium thickness and pregnancy rates in G-CSF administered COS-IUI cycles. Methods: This study was conducted in the Laxmi Narasimha hospital IVF center under the guidence Dr. Adaboina Anitha,Hanamkonda, warangal. Thin endometrium was defined less than 7mm on Trans vaginal ultrasound. Letrozole 5mg (2.5mg, BD) is used for ovulation induction on day 2 or day 3 of their cycle, G CSF 300units was added with significantly improved pregnancy rates in ART cycles. Trigger used was injection 10,000IU urinary hCG & 300 units G-CSF was instilled into the uterus. After 48hours ET was measured & IUI was performed under aseptic precautions. After 16 days β -hCG levels were monitored to determine whether there is a pregnancy. Results: In present study, we concluded that, 250 COS-IUI cycles were analysed and 50 cycles showed a thin endometrium and in them G-CSF was used. The pregnancy rate (28%) was evaluated, 14 members are positive for pregnancy. Conclusion: In our study we concluded that G-CSF and letrozole will improve increases endometrium thickness and conceiving rates in COS-IUI cycles.