Bioactive Compound Analysis and Antioxidant Activity Test on Ethanol Extract of Gotu Kola Leaves (Centella Asiatica) with Different Solvent Concentrations

Formosa Journal of Science and Technology Pub Date : 2024-02-21 DOI:10.55927/fjst.v3i2.8166

Susilawati Harahap

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Abstract

Gotu kola (Centella Asiatica) contains the active ingredients alkaloids, saponins, tannins, flavonoids, steroids and triterpenoids. Three bioactive groups, namely triterpenoids, steroids and saponins, are antioxidants that are beneficial for the health of the human body. The aim of this research is to identify the phytochemical content, total flavonoid content and antioxidant activity in gotu kola (Centella Asiatica) leaves with different solvent concentrations. The type of research is Qualitative and Quantitative with an Experimental laboratory research design carried out at the Itkessu Pharmaceutical Laboratory. The results of the phytochemical screening identified that pegagan (Centella Asiatica) leaves were positive for containing alkaloids, flavonoids, steroids, triterpenoids and saponins at both solvent concentrations. The UV-Vis results showed that the total flavonoid content in gotu kola (Centella Asiatica) leaves was 31.6271 mg QE/Gr for 70% ethanol and 21.7821 mg QE/Gr for 96% ethanol, while the IC50 value was 53.2210 ppm for 70% ethanol and 61.7269 ppm for 96% ethanol.

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不同溶剂浓度积雪草乙醇提取物的生物活性化合物分析和抗氧化活性测试

哥度柯（积雪草）含有生物碱、皂苷、单宁、类黄酮、类固醇和三萜类化合物等活性成分。三萜类、类固醇和皂苷这三种生物活性成分是抗氧化剂，对人体健康有益。本研究的目的是鉴定不同溶剂浓度的积雪草叶片中的植物化学物质含量、总黄酮含量和抗氧化活性。研究采用定性和定量相结合的实验研究设计，在 Itkessu 制药实验室进行。植物化学筛选结果表明，积雪草叶在两种溶剂浓度下均含有生物碱、黄酮类、类固醇、三萜类和皂苷。紫外可见光结果显示，70%乙醇和 96% 乙醇中的总黄酮含量分别为 31.6271 毫克 QE/Gr 和 21.7821 毫克 QE/Gr，而 70% 乙醇和 96% 乙醇中的 IC50 值分别为 53.2210 ppm 和 61.7269 ppm。

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Formosa Journal of Science and Technology

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