No evidence of the long-term in vitro toxicity of Aeroxide P25 TiO2 nanoparticles in three mammalian cell lines despite the initial reduction of cellular mitochondrial activity
IF 0.7 4区 物理与天体物理Q4 CHEMISTRY, INORGANIC & NUCLEAR
S. Męczyńska-Wielgosz, T. Bartłomiejczyk, I. Grądzka, Sylwester Sommer, Aneta Węgierek-Ciuk, A. Lankoff, Katarzyna Sikorska, M. Wojewódzka, Małgorzata M Dobrzyńska, Marcin Kruszewski
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引用次数: 0
Abstract
We studied the effects of Aeroxide P25 titanium dioxide nanoparticles (TiO2 NPs) with a diameter of 21 nm on induction of DNA damage and long-term survival of three human cell lines: hepatocellular liver carcinoma HepG2, colorectal adenocarcinoma HT29 and lung carcinoma A549. The endpoints examined were DNA breakage estimated by the comet assay and oxidative base damage recognized by formamide-pyrimidine glycosylase (FPG) estimated with the FPG+ comet assay, frequencies of histone γH2AX foci and micronuclei, apoptosis, cell metabolic activity measured by mitochondrial activity (MTT) assay and long-term survival measured by colony-forming ability. Each cell line had a different pattern of DNA breakage and base damage vs. nanoparticle (NP) concentration and treatment time. There was no increase in the frequencies of histone γH2AX foci and micronuclei as compared to those in the untreated cells. In parallel with these results, no induction of apoptosis has been found in none of the cell lines tested. The reported experiments provided no evidence of the long-term in vitro toxicity of Aeroxide P25 TiO2 NPs, despite a slight decrease in mitochondrial activity and cell survival during the first 72 h.
期刊介绍:
"Nukleonika" is an international peer-reviewed, scientific journal publishing original top quality papers on fundamental, experimental, applied and theoretical aspects of nuclear sciences.
The fields of research include:
radiochemistry, radiation measurements, application of radionuclides in various branches of science and technology, chemistry of f-block elements, radiation chemistry, radiation physics, activation analysis, nuclear medicine, radiobiology, radiation safety, nuclear industrial electronics, environmental protection, radioactive wastes, nuclear technologies in material and process engineering, radioisotope diagnostic methods of engineering objects, nuclear physics, nuclear reactors and nuclear power, reactor physics, nuclear safety, fuel cycle, reactor calculations, nuclear chemical engineering, nuclear fusion, plasma physics etc.