Identification of surfactin lipopeptides isoforms produced by a newly isolated strain Bacillus sp. MI27 with potential use as main ingredients in detergent formulation: Application of mixture design for the formulation of a bio-based detergent

Water Practice & Technology Pub Date : 2024-02-24 DOI:10.2166/wpt.2024.034

Marwa Ghoul, Mouna Bouassida, Roser Segovia, D. Ghribi, Francesc Rabanal Anglada, Mnif Inès

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Abstract

The purification and characterization of lipopeptides biosurfactants produced by Bacillus sp. MI27 permits to identify two clusters of cyclic and linear surfactin isoforms. Aiming for a potential application in industrial biotechnology, we studied its potential washing capacity. The results show a good ability to wash oil stains, motor oil and mayonnaise evaluated by the determination of the % of stain removal and tissue bleaching indices after washing. Optimal washing capacities were unregistered for 300 and 400 mg/L at pH values ranging from 5 to 9 and temperatures ranging from 20 to 40°C. Using a 4-factor mixing design, we optimized a liquid formula composed only of BioS; silicate; carboxymethyl cellulose and calcium phosphate. The washing efficiency of the formula is of the order of 43.46% for petroleum and 49.10% for motor oil with a percentage reduction in surface tension of about 55.79% corresponding to 25.7 mN/m.

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鉴定新分离菌株芽孢杆菌 MI27 产生的表面活性素脂肽异构体，这些异构体可用作洗涤剂配方的主要成分：混合设计在生物基洗涤剂配方中的应用

通过对由芽孢杆菌 MI27 产生的脂肽类生物表面活性剂的纯化和表征，我们确定了两组环状和线状的表面活性素异构体。为了将其应用于工业生物技术，我们对其潜在的洗涤能力进行了研究。结果表明，通过测定洗涤后的去污率和组织漂白指数，该菌具有良好的洗涤油渍、机油和蛋黄酱的能力。在 pH 值为 5 至 9、温度为 20 至 40°C 的条件下，300 和 400 mg/L 的最佳洗涤能力均未注册。通过 4 因子混合设计，我们优化了一种仅由 BioS、硅酸盐、羧甲基纤维素和磷酸钙组成的液体配方。该配方对石油的洗涤效率约为 43.46%，对机油的洗涤效率约为 49.10%，表面张力降低了约 55.79%，相当于 25.7 mN/m。

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Water Practice & Technology

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