Evaluation of bacteriological quality and safety of sugarcane juice locally processed and vended in Dar es Salaam City, Tanzania

Abdusudi Issa-Zacharia, Stephen Paul Rwabunywenge
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Abstract

The prevalence of foodborne illness linked to the intake of freshly squeezed juices sold by street vendors is on the rise, despite the widespread use of these beverages by millions of people in developing nations. Hence, a study was undertaken to evaluate the microbiological standard and safety of locally processed and street-vended sugarcane juices in Dar es Salaam to ascertain their present condition. A total of 60 samples of sugarcane juice were gathered and examined. Street vendors involved in the sugarcane juice business were interviewed followed by physical-chemical and microbiological laboratory analysis. The pH of unpasteurized sugarcane juice was 4.8 and 4.9 for iced and raw, respectively while the pH for pasteurized and pasteurized juice in which citric acid was added were receptively, 4.3 and 3.1. The average level of titratable acidity was 0.083%. The Soluble solids (°Brix) of unpasteurized raw, iced and pasteurized sugarcane juice ranged from 12.2- 22.1, 2.4-13.8 and 14.1-15.8. The total plate counts (TPC) of unpasteurized sugarcane juice showed a mean of 5.592 and 5.64 log cfu/mL for raw and iced sugarcane juice, respectively. About 90% of samples were above TBS and Codex recommended maximum limits of 3.7 to 4 log cfu/mL or 5×10³- 10⁴ cfu/mL. Unpasteurized raw and iced sugarcane juice were contaminated with 1.79 and 2.10 log cfu/mL of E. coli while no typical Salmonella spp. was detected in all 60 samples. The study concluded that the microbiological quality and overall handling practices associated with unpasteurized sugarcane juice sold in Dar es Salaam City were substandard.
评估坦桑尼亚达累斯萨拉姆市当地加工和销售的甘蔗汁的细菌学质量和安全性
尽管发展中国家有数百万人广泛使用街头小贩出售的鲜榨果汁,但与摄入这些饮料有关的食源性疾病发病率却在不断上升。因此,我们开展了一项研究,对达累斯萨拉姆当地加工的甘蔗汁和街头小贩出售的甘蔗汁的微生物标准和安全性进行评估,以确定它们的现状。研究人员共收集并检测了 60 份甘蔗汁样本。对从事甘蔗汁生意的街头小贩进行了访谈,然后进行了物理化学和微生物实验室分析。未经巴氏杀菌的冰甘蔗汁和生甘蔗汁的 pH 值分别为 4.8 和 4.9,而巴氏杀菌甘蔗汁和添加柠檬酸的巴氏杀菌甘蔗汁的 pH 值分别为 4.3 和 3.1。可滴定酸度的平均水平为 0.083%。未经巴氏杀菌的生甘蔗汁、冰镇甘蔗汁和巴氏杀菌甘蔗汁的可溶性固形物(°Brix)分别为 12.2-22.1、2.4-13.8 和 14.1-15.8。未经巴氏杀菌的生甘蔗汁和冰甘蔗汁的菌落总数(TPC)平均值分别为 5.592 和 5.64 log cfu/mL。约 90% 的样本高于 TBS 和 Codex 建议的最大限值 3.7 至 4 log cfu/mL 或 5×10³- 10⁴ cfu/mL。未经巴氏杀菌的生甘蔗汁和冰甘蔗汁分别被 1.79 和 2.10 log cfu/mL 的大肠杆菌污染,而在所有 60 个样本中均未检测到典型的沙门氏菌属。研究得出结论,达累斯萨拉姆市出售的未巴氏杀菌甘蔗汁的微生物质量和整体处理方法不达标。
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