Antidiabetic and Hepatoprotective Effects of Aqueous and Ethanolic Leaf Extracts of Mitracarpus Hirtus on Alloxan-Induced Diabetic Rats

Asian Journal of Science, Technology, Engineering, and Art Pub Date : 2024-02-29 DOI:10.58578/ajstea.v2i2.2775

Gabriel Sunday, Ezeonu Chukuma Stephen, Imo Chinedu, Jonah Tsuvini, Atah David Attah

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Abstract

Diabetes mellitus (DM) is a metabolic disorder that remains a major health concern worldwide. It is associated with derangement of carbohydrate, protein, and lipid metabolism. This study evaluates the antidiabetic and hepatoprotective effects of aqueous and ethanolic leaf extracts of Mitracarpus hirtus on alloxan-induced diabetic rats. Fifty-five male Wistar rats were divided into 11 groups, with diabetes induced by alloxan. Treatment with aqueous and ethanolic extracts, along with Glibenclamide, lasted three weeks. At the end of the treatment period, the rats were sacrificed and blood samples were collected via cardiac puncture for biochemical analysis. Findings revealed a significant decrease (p<0.05) in fasting blood sugar (FBS) in group 10 (99.8 ± 15.74 mg/dL) when compared with group 2 (274.2 ± 7.95 mg/dL) in week 1. Week 2 also revealed a significant decrease (p<0.05) in group 11 (84.6 ± 14.98 mg/dL) when compared with group 2 (262.8 ± 15.94 mg/dL). Week 3, further revealed a significant decrease (p<0.05) in group 9 (79.4 ± 25.13 mg/dL) and group 11 (83.6 ± 8.35 mg/dL) when compared with group 2 (289.6 ± 20.89 mg/dL). Aspartate Aminotransferase (AST) revealed a significant decrease (p<0.05) when group 11 (54.38 ± 15.74 U/L) was compared with group 2 (146.66 ± 11.35 U/L). Similarly, Alanine Aminotransferase (ALT) significantly decreased (p<0.05) when group 11 (53.46 ± 4.6 U/L) was compared with group 2 (101.42 ± 16.01 U/L), while total protein and albumin increased in all treated groups. Group 5 (0.64 ± 0.11 mg/dL) in Total bilirubin (TB) had the lowest mean when compared with group 2 (2.69 ± 0.52 mg/dL). All treatment groups significantly decreased (p<0.05) Triglyceride (TG) and Cholesterol (CHOL). From this study, it has been shown that the aqueous and ethanolic leaves extracts of Mitracarpus hirtus may have the potential to ameliorate the complications due to diabetes in a dose dependent manner.

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密蒙花水提取物和乙醇叶提取物对阿脲诱导的糖尿病大鼠的抗糖尿病和肝保护作用

糖尿病（DM）是一种代谢紊乱疾病，仍然是全球关注的主要健康问题。它与碳水化合物、蛋白质和脂质代谢紊乱有关。本研究评估了水和乙醇叶提取物对阿脲诱导的糖尿病大鼠的抗糖尿病和保肝作用。55 只雄性 Wistar 大鼠被分为 11 组，由阿脲诱导糖尿病。水提取物和乙醇提取物以及格列本脲的治疗持续三周。治疗结束后，大鼠被处死，并通过心脏穿刺采集血液样本进行生化分析。结果显示，与第 2 组（274.2 ± 7.95 mg/dL）相比，第 10 组的空腹血糖（99.8 ± 15.74 mg/dL）在第 1 周明显下降（p<0.05）。第 2 周，与第 2 组（262.8 ± 15.94 mg/dL）相比，第 11 组（84.6 ± 14.98 mg/dL）也有明显下降（p<0.05）。第 3 周，与第 2 组（289.6 ± 20.89 mg/dL）相比，第 9 组（79.4 ± 25.13 mg/dL）和第 11 组（83.6 ± 8.35 mg/dL）进一步显着下降（p<0.05）。与第 2 组（146.66 ± 11.35 U/L）相比，第 11 组（54.38 ± 15.74 U/L）的天门冬氨酸氨基转移酶（AST）明显下降（P<0.05）。同样，第 11 组（53.46 ± 4.6 U/L）与第 2 组（101.42 ± 16.01 U/L）相比，丙氨酸氨基转移酶（ALT）明显下降（P<0.05），而所有治疗组的总蛋白和白蛋白均有所增加。与第 2 组（2.69 ± 0.52 mg/dL）相比，第 5 组总胆红素（TB）的平均值最低（0.64 ± 0.11 mg/dL）。所有治疗组的甘油三酯（TG）和胆固醇（CHOL）均明显下降（P<0.05）。这项研究表明，水溶性和乙醇叶提取物具有改善糖尿病并发症的潜力，其效果与剂量有关。

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Asian Journal of Science, Technology, Engineering, and Art

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