KARAKTERISASI MORFOLOGI DAN UJI ANTIFUNGI ISOLAT JAMUR Trichoderma spp. DARI TANAH GAMBUT TERHADAP PATOGEN PADA JARAK KEPYAR (Ricinus communis L.)

Jurnal Agroteknologi Pub Date : 2024-02-29 DOI:10.24014/ja.v14i2.18479

Nurraodatul Aini, Atria Martina

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Abstract

Castor oil (Ricinus communis L.) is an oil producing commodities that plays a role in meeting industrial and economic needs. Infection with the pathogen fungi caused a decrease in the production of castor oil. Biological control using Trichoderma spp. has been widely used. Trichoderma spp. known as one of the benefical biofungicide because it has high antagonistic properties inhibiting the growth pathogenic fungi. The purpose of this study was to obtain isolates and characters as well antifungal test for Trichoderma spp. isolated from peat soil to control pathogen Aspergillus sp. on castor been (Ricinus communis L.). Trichoderma spp. isolated from peat soil in Meranti Islands, Riau. The antagonistic activity the isolates against pathogen Aspergillus sp. was studied in vitro using dual culture and in vivo assay. In this study, six isolates of Trichoderma spp have isolated. Trichoderma sp. GBA5 has the highest growth rate on PDA and TSM medium. Trichoderma sp. GBA3 and Trichoderma sp. GBA4 were most effective in percentage inhibition of mycelial growth of test pathogen i.e 84,55% and 83,71% respectively. In in vivo assay, only Trichoderma sp. GBA5 inhibited pathogen with the percent infected castor capsule was 51.66%.

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CARACTERISASI MORFOLOGI DAN UJI ANTIFUNGI ISOLAT JAMUR Trichoderma spp. DARI TANAH GAMBUT TERHADAP PATOGEN PADA JARAK KEPYAR (Ricinus communis L.)

蓖麻油（Ricinus communis L.）是一种产油商品，在满足工业和经济需求方面发挥着重要作用。病原真菌感染导致蓖麻油产量下降。利用毛霉属真菌进行生物防治已得到广泛应用。毛霉属真菌被称为有益的生物杀真菌剂之一，因为它具有抑制病原真菌生长的高度拮抗特性。本研究的目的是从泥炭土中分离出毛霉菌属，对其进行分离、特征和抗真菌测试，以控制蓖麻（Ricinus communis L.）上的病原体曲霉菌属（Aspergillus sp.）。从廖内省默兰蒂群岛泥炭土中分离出的毛霉菌属（Trichoderma spp.通过双重培养和体内试验，研究了分离物对病原曲霉的拮抗活性。在这项研究中，共分离出 6 个毛霉属分离株。毛霉 GBA5 在 PDA 和 TSM 培养基上的生长率最高。Trichoderma sp. GBA3 和 Trichoderma sp. GBA4 对测试病原体菌丝生长的抑制率最高，分别为 84.55% 和 83.71%。在体内试验中，只有 GBA5 真菌对病原体有抑制作用，其感染蓖麻蒴果的百分比为 51.66%。

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Jurnal Agroteknologi

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