CDKN1A down-regulation of inflammasomes and pro-inflammatory cytokines in pyroptosis of cartilage cells

Abstract

Osteoarthritis is the most common chronic degenerative disease worldwide; it mainly affects elderly people. This disease can involve nearly any joint in the human body, and the most common symptoms include joint pain and disordered articular functions. Inflammasomes (NLRP3) which are induced by nuclear factor kappa B (NF-κB) signaling and can convert interleukin-1β (IL-1β) and IL-18 into mature proinflammatory cytokines are considered a factor in low-grade inflammatory pathology. This study aimed to explore the mechanisms underlying CDKN1A-in osteoarthritis. Chondrocytes were collected and isolated from 22 patients with osteoarthritis (average age 50.22 ± 2.15) and healthy volunteers (average age 51.12 ± 2.34) were enrolled as the control group from June 2021 to June 2023. mRNA expression levels of CDKN1A, NLRP3, and cleaved-Caspase1) were detected by real-time PCR. Cell activity was calculated with CCK-8. It has been found that CDKN1A regulates DNA damage repair, which contributes to the improvement of osteoarthritis by regulating the pyroptosis of cartilage cells. However, the exact mechanistic effects are still unknown.