Regeneration and reuse of transwell-type culture inserts for blood-brain barrier modelling

Q3 Biochemistry, Genetics and Molecular Biology
Margarita Shuvalova, Georgii Nosov
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引用次数: 0

Abstract

Culture inserts of Transwell type are widely used in laboratories worldwide to model non-cerebral vessels, the blood-brain barrier (BBB), and other blood-tissue barriers, and also for the study of chemotaxis and transmigration. However, the use of inserts can generate plastic waste that has an environmental impact due to their one-off design and massive consumption. Thus, it is important to develop a method that can reduce the utilization of inserts but not affect research efficiency. In this study, we propose using a 1:1 (v:v) mix of 30% hydrogen peroxide and 99% sulfuric acid (“piranha solution’’) to completely remove cell debris and matrix from culture inserts. BBB models with inserts regenerated using piranha solution have barrier properties comparable to those of fresh inserts. We show that piranha solution is an effective reagent and allows for the reuse of Transwell-type inserts for BBB modeling up to 5 times. Therefore, the use of this method greatly reduces the production of laboratory waste and benefits numerous laboratories worldwide.
用于血脑屏障建模的跨孔型培养插片的再生和再利用
世界各地的实验室广泛使用 Transwell 型培养插片来模拟非脑血管、血脑屏障(BBB)和其他血-组织屏障,以及研究趋化和迁移。然而,由于插入物的一次性设计和大量消耗,使用插入物会产生对环境有影响的塑料废物。因此,开发一种既能减少插入物的使用又不影响研究效率的方法非常重要。在这项研究中,我们建议使用 30% 过氧化氢和 99% 硫酸的 1:1 (v:v)混合液("食人鱼溶液")来彻底清除培养插片上的细胞碎片和基质。使用食人鱼溶液再生插入物的 BBB 模型具有与新鲜插入物相当的屏障特性。我们的研究表明,食人鱼溶液是一种有效的试剂,可重复使用 Transwell 型插入物进行 BBB 建模达 5 次。因此,使用这种方法可以大大减少实验室废物的产生,使全球众多实验室受益。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Cellular Biotechnology
Journal of Cellular Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
0.70
自引率
0.00%
发文量
13
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