Effects of Simulated Digestion on the Bioaccessibility of Phenolic Compounds and Antioxidant Activity of Dehydrated Bignay [Antidesma bunius (L.) Spreng]
Ara Fatima A. Carbonera, L. Atienza, M. Estacio, S. M. Duque, Rona Camille M. Lizardo-Agustin, K. Castillo-Israel
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引用次数: 0
Abstract
Bigna y is a Philippine indigenous berry rich in phenolic compounds with antioxidant activity. This can be dehydrated and used as a functional ingredient, but dehydration followed by digestion results to changes in the bioactive compounds modifying their bioaccessibility. Most bioaccessibility studies are on different fresh berries and fruits while very limited on dried or processed fruits. Thus, this study investigated the effect of simulated digestion on the bioaccessibility of phenolic compounds and antioxidant activity of freeze dried, oven dried, and spray dried bignay as well as cookies with added dehydrated bignay. Total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), and antioxidant activity using 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging activity, ferric reducing antioxidant power (FRAP), and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays were determined. The TPC, TFC, TAC, and antioxidant activity of the dehydrated bignay and cookies showed significant differences (p≤ 0.05) at the end of each digestion stage. Among the dehydrated samples, the freeze dried bignay had the highest TPC, TFC, TAC and antioxidant activity after the gastric and intestinal stages resulting in a bioaccessibility of 66.39 % for TPC, 78.93 % for TFC, and 70.43 % for TAC along with a bioaccessibility of 81.45 % for DPPH, 82.28 % for FRAP, and 75.64 % for ABTS. Furthermore, the cookies with the added freeze dried bignay had the highest TPC, TFC, TAC and antioxidant activity at the end of both gastric and intestinal stages. This resulted in a bioaccessibility of 48.85 % for TPC, 47.26 % for TFC, 33.16 % for TAC, 62.60 % for DPPH, 64.39 % for FRAP, and 36.39 % for ABTS. Simulated digestion favored the release of phenolic compounds with antioxidant activity, although the contents were lower than that obtained before the simulated digestion. The total phenolics, flavonoids, anthocyanin, and antioxidant activity were better preserved by freeze drying followed by oven drying and least by spray drying.
期刊介绍:
The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.