Fenugreek Seed Extract Mitigates MSG-Induced Uterine Dysfunction in Rats through Enhanced Antioxidative Defense Mechanisms

Abstract

To assess the mitigating effectiveness of fenugreek seed extract in MSG-induced suppression of uterine function in rats, the effects of MSG in combination with fenugreek seed extract on the oxidative stress variables in the uterus and histo-architectural changes in the uterus have been studied and compared with the results obtained in the uterus in MSG-treated and control groups of rats. The study investigated the impact of MSG in conjunction with fenugreek seed extract on uterine function and oxidative stress. Female virgin albino rats were distributed  into seven groups (Control, Treated I, Treated II, Treated III, Treated I+ Fenugreek seed extract, Treated II+ Fenugreek seed extract, Treated III+ Fenugreek seed extract) and subjected to thirty-day and forty-day of treatment via oral gavage. Despite no significant changes in mean body weight observed in MSG and fenugreek seed extract combination groups compared to the control, a notable counteraction was observed in the results obtained from rats exposed to MSG alone. This implies a possible protective function of fenugreek seed extract against oxidative stress in the uterus caused by MSG. Rats that received MSG in combination with fenugreek seed extract in their uterine tissue homogenate showed negligible changes in the activities of SOD, CAT, GR, GPx, GST, and MDA production when compared to the control group. Additionally, when comparing the MSG-exposed groups compare to control groups, a noteworthy decline in the activities of SOD, CAT, GR, and GPx and an increase in the activity of GST and MDA production were noted. When MSG was applied in conjunction with fenugreek seed extract for both treatment durations, no discernible histo-architectural alterations in the uterus were seen compared to the uterine tissues of the control groups of rats. Thus, it can be inferred from the findings that fenugreek seed extract significantly mitigated the oxidative stress and histo-architectural changes in rat uterine tissues caused by MSG.