Immunohistochemical localization and expression of heat shock proteins (HSP27, HSP60, HSP70, and HSP90) during the oestrous cycle, pregnancy, and lactation in rat ovaries

IF 2.3 4区生物学 Q4 CELL BIOLOGY

Acta histochemica Pub Date : 2024-04-01 DOI:10.1016/j.acthis.2024.152157

Banu Kandil , Nevin Kurtdede , Alev Gürol Bayraktaroglu

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引用次数: 0

Abstract

This study aimed to determine the expressions of HSP27, HSP60, HSP70, and HSP90 in rat ovaries during the oestrous cycle, pregnancy, and lactation. In follicle cells, HSP27 and HSP70 expression was not observed. HSP60 in oocytes was higher in the early stages of follicular development but decreased and disappeared as the follicle grew. HSP60 in granulosa and theca cells increased with follicle development and decreased with atresia. HSP90 in follicle cells did not change during follicle development or atresia. The expression of HSPs in interstitial cells was higher in the proestrus and estrus phases of the estrous cycle. The expression of HSPs in these cells was higher on day 5 of pregnancy, decreased on day 10, and decreased further on days 15 and 20. The expression of HSPs, which decreased in the second half of pregnancy, increased again on the first day of lactation. The expression of HSPs then decreased on day 5 of lactation and further decreased on days 10 and 20. HSP60 and HSP90 were positive in new and old corpus luteums (CLs) and their expression did not change during luteal development or regression. HSP27 and HSP70 were absent in new CLs. HSP27 was positive in old CLs and showed the same staining pattern during luteal regression. HSP70 expression was determined in old cyclic CLs during the oestrous cycle and pregnancy and decreased with luteal regression. HSP70 expression in old pregnancy CLs during lactation was very weak compared to the oestrous cycle and pregnancy. In conclusion, HSP60 and HSP90 may participate in folliculogenesis, luteal development, and steroidogenesis in luteal cells, and HSP27, HSP60, HSP70, and HSP90 may be effective in luteal regression and steroidogenesis in interstitial cells. HSP27 and HSP70 may be used as markers to identify old CLs in rats.

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大鼠卵巢发情周期、妊娠和哺乳期热休克蛋白（HSP27、HSP60、HSP70 和 HSP90）的免疫组织化学定位和表达

本研究旨在确定发情周期、妊娠期和哺乳期大鼠卵巢中 HSP27、HSP60、HSP70 和 HSP90 的表达情况。在卵泡细胞中，未观察到 HSP27 和 HSP70 的表达。卵泡发育早期，卵母细胞中的 HSP60 表达量较高，但随着卵泡的长大，HSP60 的表达量逐渐降低并消失。颗粒细胞和癌细胞中的 HSP60 随卵泡发育而增加，随闭锁而减少。卵泡细胞中的HSP90在卵泡发育或闭锁期间没有变化。在发情周期的预发情期和发情期，间质细胞中 HSPs 的表达量较高。这些细胞中 HSPs 的表达量在妊娠第 5 天较高，第 10 天下降，第 15 天和第 20 天进一步下降。妊娠后半期，HSPs 的表达量有所下降，但在哺乳期的第一天，HSPs 的表达量又有所增加。随后，HSPs 的表达在哺乳期第 5 天下降，在第 10 天和第 20 天进一步下降。HSP60和HSP90在新黄体和旧黄体中均呈阳性，其表达在黄体发育或退化过程中没有变化。新黄体中没有 HSP27 和 HSP70。HSP27在老黄体中呈阳性，并在黄体退化过程中显示出相同的染色模式。HSP70在发情周期和妊娠期的旧周期性CL中表达，并随着黄体退化而减少。与发情周期和妊娠期相比，哺乳期妊娠期CL中HSP70的表达非常弱。总之，HSP60和HSP90可能参与黄体细胞的卵泡生成、黄体发育和类固醇生成，而HSP27、HSP60、HSP70和HSP90可能对黄体退化和间质细胞的类固醇生成有效。HSP27 和 HSP70 可用作鉴定大鼠老旧 CL 的标记物。

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来源期刊

Acta histochemica 生物-细胞生物学

CiteScore

4.60

自引率

4.00%

发文量

107

审稿时长

23 days

期刊介绍： Acta histochemica, a journal of structural biochemistry of cells and tissues, publishes original research articles, short communications, reviews, letters to the editor, meeting reports and abstracts of meetings. The aim of the journal is to provide a forum for the cytochemical and histochemical research community in the life sciences, including cell biology, biotechnology, neurobiology, immunobiology, pathology, pharmacology, botany, zoology and environmental and toxicological research. The journal focuses on new developments in cytochemistry and histochemistry and their applications. Manuscripts reporting on studies of living cells and tissues are particularly welcome. Understanding the complexity of cells and tissues, i.e. their biocomplexity and biodiversity, is a major goal of the journal and reports on this topic are especially encouraged. Original research articles, short communications and reviews that report on new developments in cytochemistry and histochemistry are welcomed, especially when molecular biology is combined with the use of advanced microscopical techniques including image analysis and cytometry. Letters to the editor should comment or interpret previously published articles in the journal to trigger scientific discussions. Meeting reports are considered to be very important publications in the journal because they are excellent opportunities to present state-of-the-art overviews of fields in research where the developments are fast and hard to follow. Authors of meeting reports should consult the editors before writing a report. The editorial policy of the editors and the editorial board is rapid publication. Once a manuscript is received by one of the editors, an editorial decision about acceptance, revision or rejection will be taken within a month. It is the aim of the publishers to have a manuscript published within three months after the manuscript has been accepted