Imaging Neuropeptide Release from Drosophila Clock and Motor Neurons.

Cold Spring Harbor protocols Pub Date : 2025-03-03 DOI:10.1101/pdb.top107798

Edwin S Levitan, Dinara Bulgari, Markus K Klose

引用次数: 0

Abstract

Electrophysiological studies of synaptic function do not robustly report release of neuropeptides and neurotrophins. These limitations have been overcome with the presynaptic expression of optical release reporters based on green fluorescent protein and fluorogen-activating protein. Here we describe how to image neuropeptide release in Drosophila at the neuromuscular junction and in the adult brain.

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果蝇时钟和运动神经元神经肽释放成像

突触功能的电生理研究并不能有力地报告神经肽和神经营养素的释放。通过在突触前表达基于绿色荧光蛋白和荧光激活蛋白的光学释放报告，这些局限性得以克服。在这里，我们描述了如何对果蝇神经肌肉接头处和成虫大脑中的神经肽释放进行成像。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cold Spring Harbor protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.00

自引率

0.00%

发文量

163

期刊介绍： Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Cold Spring Harbor Protocols is an interdisciplinary journal providing a definitive source of research methods in cell, developmental and molecular biology, genetics, bioinformatics, protein science, computational biology, immunology, neuroscience and imaging. Each monthly issue details multiple essential methods—a mix of cutting-edge and well-established techniques.