Cytogenetic analysis reveals a mechanism of compatibility in distant hybridization between tree peony and herbaceous peony

IF 1.6 3区农林科学 Q2 AGRONOMY

Euphytica Pub Date : 2024-03-28 DOI:10.1007/s10681-024-03326-1

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引用次数: 0

Abstract

Cross-incompatibility is the major challenge in inter-sectional distant hybridization between tree peonies (Paeonia sect. Moutan) and herbaceous peonies (Paeonia sect. Paeonia). Most of the intersectional cross combinations rarely produce hybrid progenies or even seeds. However, a combination with Paeonia × lemoinei Rehd. (P. delavayi × P. suffruticosa, tree type) as the pollen parent and P. lactiflora (herbaceous) as the seed parent resulted in many progenies. This research focuses on cytogenetics of two highly compatible intersectional combinations, P. lactiflora ‘Martha W.’ × P. × lemoinei ‘Golden Era’ (‘MW’ × ‘GE’) and P. lactiflora ‘Fen Yun Fei He’ × P. × lemoinei ‘L’Esperance’ (‘FYFH’ × ‘LE’). Karyotype analysis combined with genomic in situ hybridization (GISH) showed that the seed parents, ‘MW’ and ‘FYFH’, are both diploids (2n = 2x = 10, CC), while the pollen parents, ‘GE’ and ‘LE’, are tetraploid (2n = 4x = 20, AABB) and diploid (2n = 2x = 10, AB) respectively. Four progenies from ‘MW’ × ‘GE’, and another four progenies from ‘FYFH’ × ‘LE’, are all triploids (2n = 3x = 15, ABC). P. × lemoinei contributes two sets of chromosomes (AB) showing distinct signals in all tested triploids. The essential of a successful intersectional hybridization is to obtain 2x gametes from tree peony. P. × lemoinei has better compatibility in intersectional hybridization than other tree peony species, probably because of its higher frequency of 2x gamete (unreduced in diploid, or reduced in tetraploid) generation. Tetraploid tree peonies, such as ‘GE’, might produce abundant 2x gametes through normal meiosis, which is the possible reason for their better fertility and superior intersectional compatibility than diploid tree peonies. The mechanism of compatibility in intersectional hybridization is supposed to be a rebalance between the distant divergent parents, tree peonies and herbaceous peonies, triggered by ploidy increase of gametes from tree peonies. The discovery of tetraploid tree peony also provides valuable materials to study the mechanism of polyploidization in sect. Moutan, and to promote the distant hybridization breeding in Paeonia.

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细胞遗传学分析揭示了树牡丹与草本牡丹远缘杂交的相容性机制

摘要交配不亲和是树牡丹（牡丹科）和草本牡丹（芍药科）间远缘杂交的主要挑战。大多数节间杂交组合很少产生杂交后代甚至种子。然而，以 Paeonia × lemoinei Rehd.（P. delavayi × P. suffruticosa，乔木型）为花粉亲本，以 P. lactiflora（草本）为种子亲本的组合却产生了许多后代。本研究的重点是两个高度相容的交叉组合的细胞遗传学，即 P. lactiflora 'Martha W.' × P.× lemoinei 'Golden Era'（'MW' × 'GE'）和 P. lactiflora 'Fen Yun Fei He' × P.× lemoinei 'L'Esperance' （'FYFH' × 'LE'）。核型分析结合基因组原位杂交（GISH）显示，种子亲本'MW'和'FYFH'都是二倍体（2n = 2x = 10，CC），而花粉亲本'GE'和'LE'分别是四倍体（2n = 4x = 20，AABB）和二倍体（2n = 2x = 10，AB）。MW'×'GE'的四个后代和'FYFH'×'LE'的另外四个后代都是三倍体（2n = 3x = 15，ABC）。P. × lemoinei 有两组染色体（AB），在所有测试的三倍体中都显示出不同的信号。成功交叉杂交的关键在于从树牡丹中获得 2x 配子。P. × lemoinei 在交叉杂交中比其他树牡丹品种具有更好的兼容性，这可能是因为其产生 2 倍配子（在二倍体中为未还原配子，在四倍体中为还原配子）的频率较高。四倍体牡丹（如 "GE"）可能通过正常的减数分裂产生大量的 2 倍配子，这可能是它们比二倍体牡丹繁殖力更强、交叉相容性更好的原因。相交杂交的相容性机制应该是树牡丹和草本牡丹这对远缘亲本之间的再平衡，由树牡丹配子倍性的提高引发的。四倍体树牡丹的发现也为研究牡丹科的多倍体化机制提供了宝贵的材料。四倍体树牡丹的发现也为研究牡丹科的多倍体机制、促进牡丹远缘杂交育种提供了宝贵材料。

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来源期刊

Euphytica 农林科学-农艺学

CiteScore

3.80

自引率

5.30%

发文量

157

审稿时长

4.5 months

期刊介绍： Euphytica is an international journal on theoretical and applied aspects of plant breeding. It publishes critical reviews and papers on the results of original research related to plant breeding. The integration of modern and traditional plant breeding is a growing field of research using transgenic crop plants and/or marker assisted breeding in combination with traditional breeding tools. The content should cover the interests of researchers directly or indirectly involved in plant breeding, at universities, breeding institutes, seed industries, plant biotech companies and industries using plant raw materials, and promote stability, adaptability and sustainability in agriculture and agro-industries.