Unraveling the microbial status of wild bee’s honey from North India

IF 1.4 4区生物学 Q3 BIOLOGY

Biologia Pub Date : 2024-03-28 DOI:10.1007/s11756-024-01655-z

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引用次数: 0

Abstract

To the best of our knowledge, this paper presents the first report on the microbial status of wild bees (A. dorsata and A. florea) honey collected from different northern states of India. In total, 17 bacterial morphotypes (9 from A. dorsata and 8 from A. florea) were recovered. The bacterial load (log CFU/g) was observed to be maximum (4.01) in A. dorsata honey from Sirmour-Puruwala whereas, lowest load (3.49) was recorded in Punjab-Rupnagar honey. Likewise, A. florea honey from Sirmour-Kala Amb and Punjab-Doraha were recorded with maximum and minimum bacterial loads of 4.17 and 3.40, respectively. Observance of lower bacterial load than hazardous limit ensured its safety for human consumption. Three most dominant bacteria viz., AD1, HF7 and AF4 were identified as Micrococcus endophyticus (MT938911), Bacillus subtilis subsp. stercoris (MT764923) and Bacillus pumilus (MT764923), respectively through 16S rRNA ribotyping. Moreover, the inhibitory activity of honey was good to excellent against Salmonella typhi NCTC 786 and fair to good against Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa ATCC 10662 and Klebsiella pneumoniae ATCC 13883. The Escherichia coli ATCC 1041 was the most sensitive bacterium among all. These results justify wild bees honey as a natural remedy for curing and preventing numerous human ailments.

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揭示北印度野生蜜蜂蜂蜜的微生物状况

摘要据我们所知，本文首次报告了从印度北部各邦采集的野生蜜蜂（A. dorsata 和 A. florea）蜂蜜的微生物状况。总共回收了 17 种细菌形态（9 种来自 A. dorsata，8 种来自 A. florea）。据观察，来自 Sirmour-Puruwala 的 A. dorsata 蜂蜜的细菌量（log CFU/g）最高（4.01），而 Punjab-Rupnagar 蜂蜜的细菌量最低（3.49）。同样，来自 Sirmour-Kala Amb 和 Punjab-Doraha 的 A. florea 蜂蜜的最大和最小细菌量分别为 4.17 和 3.40。这些蜂蜜的含菌量低于危险限值，可确保人类食用安全。通过 16S rRNA 核糖体分型，确定了三种最主要的细菌，即 AD1、HF7 和 AF4，它们分别是内生微球菌（MT938911）、枯草芽孢杆菌亚种（MT764923）和枯草芽孢杆菌（MT764923）。此外，蜂蜜对伤寒沙门氏菌 NCTC 786 的抑制活性为良好至极佳，对枯草芽孢杆菌 ATCC 6633、铜绿假单胞菌 ATCC 10662 和肺炎克雷伯菌 ATCC 13883 的抑制活性为一般至良好。大肠杆菌 ATCC 1041 是最敏感的细菌。这些结果证明野蜂蜂蜜是治疗和预防人类多种疾病的天然药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biologia 生物-生物学

CiteScore

3.30

自引率

6.70%

发文量

290

审稿时长

6 months

期刊介绍： Established in 1946, Biologia publishes high-quality research papers in the fields of microbial, plant and animal sciences. Microbial sciences papers span all aspects of Bacteria, Archaea and microbial Eucarya including biochemistry, cellular and molecular biology, genomics, proteomics and bioinformatics. Plant sciences topics include fundamental research in taxonomy, geobotany, genetics and all fields of experimental botany including cellular, whole-plant and community physiology. Zoology coverage includes animal systematics and taxonomy, morphology, ecology and physiology from cellular to molecular level.