Novel species and new records of Diaporthe causing eggplant leaf and fruit blight in the Philippines

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-03-25 DOI:10.1007/s11557-024-01957-1

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引用次数: 0

Abstract

Fungal pathogens pose threats to crop productivity and food security. Management of fungal diseases can be achieved through an integrated disease management approach, including accurate identification of the causative agents. Diaporthe blight is a common disease of eggplant (Solanum melongena). Although Diaporthe vexans has been implicated as the causative agent, it was unclear until this study whether other Diaporthe species also contribute to this disease. In this study, leaf and fruit blights of eggplants were collected from different provinces in the Philippines. Through a polyphasic approach—morphological, cultural, pathogenicity, and multi-locus phylogenetic analyses of ITS, TEF1-α, TUB2, CAL, and HIS3 coupled with genealogical concordance phylogenetic species recognition analyses—we identified six Diaporthe speciesfrom the D. sojae species complex and D. arecae species complex. Two novel species, D. solani-melongenae and D. talong, along with the re-validated D. melongenae, and known species, viz., D. arecae, D. passifloricola, and D. vexans were identified as the causative agents of Diaporthe leaf and fruit blight of eggplant in the Philippines. Additionally, we also provide evidence supporting the synonymization of D. etinsidea with D. tulliensis, D. durionigena with D. rosae, and D. griceae with D. vexans. Our study confirmed that D. vexans is the major causative agent of eggplant blight in the country. All Diaporthe species were found to be pathogenic to eggplant. The results of this study contribute to the understanding of the eggplant blight disease, its potential spread, and the development of more targeted management strategies.

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菲律宾引起茄子叶枯病和果实枯萎病的 Diaporthe 新物种和新记录

摘要真菌病原体对作物生产力和粮食安全构成威胁。真菌病害的防治可通过综合病害防治方法来实现，包括准确识别致病原。Diaporthe blight 是茄子（Solanum melongena）的一种常见病。虽然 Diaporthe vexans 被认为是病原菌，但在本研究之前，还不清楚其他 Diaporthe 种类是否也会导致这种病害。本研究从菲律宾不同省份收集了茄子的叶枯病和果枯病病原。通过形态学、文化、致病性、ITS、TEF1-α、TUB2、CAL 和 HIS3 的多焦点系统发育分析以及系谱一致的系统发育物种识别分析等多相方法，我们从 D. sojae 物种复合体和 D. arecae 物种复合体中确定了六个 Diaporthe 物种。两个新种（D. solani-melongenae和D. talong）以及重新验证的D. melongenae和已知种（即D. arecae、D. passifloricola和D. vexans）被确定为菲律宾茄子Diaporthe叶枯病和果枯病的病原菌。此外，我们还提供了支持 D. etinsidea 与 D. tulliensis、D. durionigena 与 D. rosae 和 D. griceae 与 D. vexans 同名的证据。我们的研究证实，D. vexans 是该国茄子枯萎病的主要病原体。所有 Diaporthe 种类都对茄子具有致病性。这项研究的结果有助于人们了解茄子枯萎病、其潜在的传播途径以及制定更有针对性的管理策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464