Manon Dechêne-Tempier, Claire de Boisséson, Pierrick Lucas, Stéphanie Bougeard, Virginie Libante, Corinne Marois-Créhan, Sophie Payot
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引用次数: 0
Abstract
Streptococcus suis is a leading cause of infection in pigs, causing extensive economic losses. In addition, it can also infect wild fauna, and can be responsible for severe infections in humans. Increasing antimicrobial resistance (AMR) has been described in S. suis worldwide and most of the AMR genes are carried by mobile genetic elements (MGEs). This contributes to their dissemination by horizontal gene transfer. A collection of 102 strains isolated from humans, pigs and wild boars in France was subjected to whole genome sequencing in order to: (i) study their genetic diversity, (ii) evaluate their content in virulence-associated genes, (iii) decipher the mechanisms responsible for their AMR and their association with MGEs, and (iv) study their ability to acquire extracellular DNA by natural transformation. Analysis by hierarchical clustering on principal components identified a few virulence-associated factors that distinguish invasive CC1 strains from the other strains. A plethora of AMR genes (n=217) was found in the genomes. Apart from the frequently reported erm(B) and tet(O) genes, more recently described AMR genes were identified [vga(F)/sprA, vat(D)]. Modifications in PBPs/MraY and GyrA/ParC were detected in the penicillin- and fluoroquinolone-resistant isolates respectively. New AMR gene-MGE associations were detected. The majority of the strains have the full set of genes required for competence, i.e for the acquisition of extracellular DNA (that could carry AMR genes) by natural transformation. Hence the risk of dissemination of these AMR genes should not be neglected.
猪链球菌是猪感染的主要原因,造成了巨大的经济损失。此外,它还能感染野生动物,并能导致人类的严重感染。在全球范围内,猪链球菌的抗菌药耐药性(AMR)不断增加,而大多数 AMR 基因都是由移动遗传因子(MGEs)携带的。 这有助于它们通过水平基因转移进行传播。对从法国人、猪和野猪中分离出来的 102 株菌株进行了全基因组测序,目的是(i)研究其遗传多样性,(ii)评估其毒力相关基因的含量,(iii)破译导致其 AMR 及其与 MGEs 关联的机制,(iv)研究其通过自然转化获得胞外 DNA 的能力。通过对主成分进行分层聚类分析,发现了一些与毒力相关的因子,这些因子将侵袭性 CC1 菌株与其他菌株区分开来。在基因组中发现了大量的 AMR 基因(n=217)。除了经常报道的erm(B)和tet(O)基因外,还发现了最近描述的AMR基因[vga(F)/sprA、vat(D)]。在耐青霉素和耐氟喹诺酮的分离物中分别检测到了 PBPs/MraY 和 GyrA/ParC 的修饰。发现了新的 AMR 基因-MGE 关联。大多数菌株都有能力所需的全套基因,即通过自然转化获得细胞外 DNA(可携带 AMR 基因)。因此,这些 AMR 基因的传播风险不容忽视。
期刊介绍:
Microbial Genomics (MGen) is a fully open access, mandatory open data and peer-reviewed journal publishing high-profile original research on archaea, bacteria, microbial eukaryotes and viruses.