Modulatory influence of Parkia biglobosa protein isolate on testosterone and biomarkers of oxidative stress in brain and testes of streptozotocin-induced diabetic male rats.

International journal of physiology, pathophysiology and pharmacology Pub Date : 2016-09-30 eCollection Date: 2016-01-01
Bolajoko Idiat Ogunyinka, Babatunji Emmanuel Oyinloye, Foluso Oluwagbemiga Osunsanmi, Andrew Rowland Opoku, Abidemi Paul Kappo
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Abstract

Parkia biglobosa seed an important household spice commonly consumed in Nigeria is believed to possess antioxidant activity that may exert modulatory effects in diabetes and diabetic complications. This study investigated the modulatory potential of Parkia biglobosa protein isolate (PBPi) on serum testosterone (sTT) level as well as its influence on biomarkers of oxidative stress in brain and testes of streptozotocin-induced diabetic male rats. Animals were made diabetic by single intraperitoneal administration of streptozotocin (STZ; 60 mg/kg body weight). PBPi (200 or 400 mg/kg body weight) was given orally by gavage or insulin (5 U/kg, i.p.) was administered daily to STZ-induced diabetic rats for 28 days. The results revealed a significant elevation in thiobarbituric acid reactive substances (TBARS) levels in the brain and testes of diabetic rats. This was closely associated with a concomitant reduction in levels of sTT and reduced testes weight, a noticeable decline in the glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) as well as total glutathione (Total GSH) level in the brain and testes of diabetic rats. Interestingly, treatment with PBPi efficiently prevented the alterations witnessed in the serum sTT and also ameliorated various alterations in the biomarkers of oxidative stress (TBARS, Total GSH, GST, SOD and CAT) in brain and testes of diabetic rats. These results provide evidence that PBPi could protect the brain and testicular tissues against oxidative stress induced by STZ, via modulation of serum testosterone concentration and also by enhancing antioxidant defence system in STZ-diabetic rats.

大叶朴树蛋白分离物对链脲佐菌素诱导的糖尿病雄性大鼠大脑和睾丸中睾酮和氧化应激生物标志物的调节作用
大叶朴树籽是尼日利亚人经常食用的一种重要家用香料,据信具有抗氧化活性,可对糖尿病和糖尿病并发症产生调节作用。本研究调查了大叶朴树分离蛋白(PBPi)对血清睾酮(sTT)水平的调节潜力,以及它对链脲佐菌素诱导的糖尿病雄性大鼠大脑和睾丸氧化应激生物标志物的影响。通过腹腔注射链脲佐菌素(STZ;60 毫克/千克体重)使动物患上糖尿病。给 STZ 诱导的糖尿病大鼠灌胃口服 PBPi(200 或 400 毫克/千克体重)或每天注射胰岛素(5 U/kg,静注),连续 28 天。结果显示,糖尿病大鼠脑部和睾丸中硫代巴比妥酸反应物质(TBARS)水平明显升高。与此同时,糖尿病大鼠大脑和睾丸中的 sTT 水平降低、睾丸重量减轻、谷胱甘肽-S-转移酶(GST)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)以及总谷胱甘肽(Total GSH)水平明显下降。有趣的是,使用 PBPi 能有效防止血清 sTT 的变化,还能改善糖尿病大鼠大脑和睾丸中氧化应激生物标志物(TBARS、总 GSH、GST、SOD 和 CAT)的各种变化。这些结果证明,PBPi 可通过调节血清睾酮浓度和增强 STZ 糖尿病大鼠的抗氧化防御系统,保护大脑和睾丸组织免受 STZ 诱导的氧化应激。
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