Effects of quercetin on gentamicin-induced experimental testicular injury in rats.

IF 1.2 4区 医学 Q4 DEVELOPMENTAL BIOLOGY
Ayse Firuze Biyik, Esin Yulug, Engin Yenilmez, Abdulkadir Kutlu, Ahmet Alver, Seniz Erdem
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引用次数: 0

Abstract

The purpose of this study was to investigate the effects of gentamicin (GEN) on the testis and whether quercetin (QUE) has any protective effect. Twenty-four adult male Sprague-Dawley rats were divided into equal four groups: control (0.9% saline solution), GEN (80 mg∕kg GEN), QUE (50 mg∕kg QUE) and GEN+QUE (80 mg∕kg GEN + 50 mg∕kg QUE). Histopathological (HP) evaluation of testis was performed, epididymal sperm parameters were analyzed and oxidative status was evaluated. The use of QUE improved the HP findings, such as decrease in the germinal epithelial thickness in the testicular tissue of the GEN group, decrease in the Johnsen's tubular biopsy score (JTBS), increase in the rate of immature cell shedding tubules, and the apoptotic index (AI). In the GEN group, sperm count, and abnormal morphology increased compared to the control group; the viability and motility decreased according to the sperm analysis results. In the GEN+QUE group, QUE was found to improve sperm viability and morphology. In the GEN group, tissue malondialdehyde (MDA) levels increased while superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) levels decreased. Compared with the GEN+QUE group, it was found that the tissue MDA level decreased, while the levels of SOD, CAT and GPx increased. The results demonstrate that GEN impairs testicular structure and function, and QUE treatment can prevent this adverse effect.

槲皮素对庆大霉素诱导的大鼠实验性睾丸损伤的影响
本研究旨在探讨庆大霉素(GEN)对睾丸的影响以及槲皮素(QUE)是否具有保护作用。将 24 只成年雄性 Sprague-Dawley 大鼠分为四组:对照组(0.9% 生理盐水)、GEN 组(80 mg∕kg GEN)、QUE 组(50 mg∕kg QUE)和 GEN+QUE 组(80 mg∕kg GEN + 50 mg∕kg QUE)。对睾丸进行了组织病理学(HP)评估,分析了附睾精子参数,并评估了氧化状态。QUE 的使用改善了组织病理学评估结果,如 GEN 组睾丸组织的生精上皮厚度减少、Johnsen 肾小管活检评分(JTBS)降低、未成熟细胞脱落肾小管率和细胞凋亡指数(AI)增加。与对照组相比,GEN 组的精子数量和异常形态均有所增加;根据精子分析结果,其存活率和活力均有所下降。在 GEN+QUE 组中,QUE 可提高精子活力和形态。在 GEN 组中,组织丙二醛 (MDA) 水平升高,而超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT) 和谷胱甘肽过氧化物酶 (GPx) 水平降低。结果发现,与 GEN+QUE 组相比,组织 MDA 水平下降,而 SOD、CAT 和 GPx 水平上升。结果表明,GEN 会损害睾丸的结构和功能,而 QUE 治疗可以防止这种不良影响。
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来源期刊
CiteScore
1.70
自引率
20.00%
发文量
221
审稿时长
3-8 weeks
期刊介绍: Romanian Journal of Morphology and Embryology (Rom J Morphol Embryol) publishes studies on all aspects of normal morphology and human comparative and experimental pathology. The Journal accepts only researches that utilize modern investigation methods (studies of anatomy, pathology, cytopathology, immunohistochemistry, histochemistry, immunology, morphometry, molecular and cellular biology, electronic microscopy, etc.).
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