Evaluation of sensitivity of Extended Gate Field Effect Transistor -biosensor based on V2O5/GOx for glucose detection

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2024-03-16 DOI:10.1016/j.enzmictec.2024.110428

Alessandra Teixeira Felix , Marcelo Mulato , Elidia Maria Guerra

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Abstract

The sensing modified electrode was prepared using glucose oxidase immobilized onto vanadium pentoxide xerogel with glass/FTO as support electrode to evaluate the possibility to construct a V₂O₅/GOx Extended Gate Field Effect Transistor biosensor. Previously, our studies exhibited a sensitivity of V₂O₅ of 58.1 mV/pH. The use of Nafion® onto V₂O₅/GOx caused a decrease of mass loss after several cycles compared to the modified electrode without Nafion® during the EQCM and cyclic voltammetrics studies. Electrical characterization of V₂O₅/GOx demonstrated a tendency to stability after 200 s as a function of applied current. In presence of glucose and in different pH, the current decreased when the glucose concentration increased due to the lower active sites of enzyme. After ten voltammetric cycles, the total charge tends to structural stability. In pH = 5.0, the modified electrode based on V₂O₅/GOx Extended Gate Field Effect Transistor presented more tendency to sensitivity in different concentration of glucose.

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评估基于 V2O5/GOx 的扩展栅场效应晶体管生物传感器检测葡萄糖的灵敏度

为了评估构建 V2O5/GOx 扩展栅场效应晶体管生物传感器的可能性，我们使用固定在五氧化二钒异凝胶上的葡萄糖氧化酶制备了传感修饰电极，并用玻璃/FTO 作为支撑电极。此前，我们的研究显示 V2O5 的灵敏度为 58.1 mV/pH。在 EQCM 和循环伏安法研究中，在 V2O5/GOx 上使用 Nafion® 与不使用 Nafion® 的修饰电极相比，经过几个循环后质量损失有所减少。V2O5/GOx 的电学特性表明，在 200 秒后，其稳定性与施加的电流成函数关系。在葡萄糖存在和不同 pH 值的情况下，由于酶的活性位点较低，当葡萄糖浓度增加时，电流下降。经过十次伏安循环后，总电荷趋于结构稳定。在 pH = 5.0 的条件下，基于 V2O5/GOx 扩展栅场效应晶体管的改良电极在不同浓度的葡萄糖中表现出更高的灵敏度。

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.