Certification of a novel matrix reference material for accurate measurements of aflatoxin M1 in milk powder

IF 0.8 4区工程技术 Q4 CHEMISTRY, ANALYTICAL

Accreditation and Quality Assurance Pub Date : 2024-03-13 DOI:10.1007/s00769-023-01567-6

Chun Yuan Huang, Ya Xuan Liu, Jian Zhou, Ming Wang, Meng Rui Yang, Hui Liu, Fukai Li, Liyuan Zhang

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Abstract

In this study, two different concentrations of matrix certified reference materials (CRMs) were produced for the accurate measurement of aflatoxin M1(AFM1) in milk powder (GBW(E) 100552, GBW(E) 100553). The raw material was obtained by feeding cows with positive drugs. The homogeneity, stability and characterization of these matrix CRMs were examined by liquid chromatography tandem mass spectrometry with isotope-labeled internal standard method. The certified value for the low concentration of AFM1 in milk powder was 2.45 µg/kg with an expanded uncertainty of 0.43 µg/kg (coverage factor k = 2, at 95% confidence). The certified value for the high concentration of AFM1 in milk powder was 3.45 µg/kg with an expanded uncertainty of 0.49 µg/kg (coverage factor k = 2, at 95% confidence). In addition, the samples were evaluated in detail for homogeneity, long-term stability at − 80 °C for 6 months and short-term stability at 4 °C for 7 days. The results showed that the samples were stable and homogeneous under the above conditions.

Graphical abstract

Abstract Image

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认证用于精确测量奶粉中黄曲霉毒素 M1 的新型基质标准物质

本研究采用两种不同浓度的基质有证标准物质（GBW(E) 100552 和 GBW(E) 100553），用于准确测定奶粉中的黄曲霉毒素 M1（AFM1）。原料是用阳性药物喂养奶牛获得的。采用液相色谱串联质谱法和同位素标记内标法检测了这些基质 CRMs 的均一性、稳定性和特征。奶粉中低浓度 AFM1 的认证值为 2.45 µg/kg，不确定度为 0.43 µg/kg（覆盖因子 k = 2，置信度为 95%）。奶粉中 AFM1 的高浓度认证值为 3.45 µg/kg，不确定度为 0.49 µg/kg（覆盖因子 k = 2，置信度为 95%）。此外，还对样品的均匀性、在 - 80 °C 下 6 个月的长期稳定性和在 4 °C 下 7 天的短期稳定性进行了详细评估。结果表明，在上述条件下，样品是稳定和均匀的。

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来源期刊

Accreditation and Quality Assurance 工程技术-分析化学

CiteScore

1.80

自引率

22.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Accreditation and Quality Assurance has established itself as the leading information and discussion forum for all aspects relevant to quality, transparency and reliability of measurement results in chemical and biological sciences. The journal serves the information needs of researchers, practitioners and decision makers dealing with quality assurance and quality management, including the development and application of metrological principles and concepts such as traceability or measurement uncertainty in the following fields: environment, nutrition, consumer protection, geology, metallurgy, pharmacy, forensics, clinical chemistry and laboratory medicine, and microbiology.