Cytological, Histopathological and Immunohistochemical Studies on Naturally Occurring Cutaneous Melanomas in Cattle in and Around Namakkal, Tamil Nadu, India

IF 0.4 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Indian Journal of Animal Research Pub Date : 2024-03-16 DOI:10.18805/ijar.b-5035

P. Nithya, G. Balasubramaniam, A. Arulmozhi, T. Gopalakrishnamurthy, S. Kathirvel

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引用次数: 0

Abstract

Background: Melanomas are tumours that originate from melanoblasts. The studies on bovine tumours are of clinical concern since some melanocytic tumours are congenital and occur in young animals. The present work was carried out to study the pathomorphological characteristics of melanomas in cattle for early diagnosis and intervention. Methods: Tumour samples were collected from suspected cases of neoplasia from four animals during the study period. Data pertaining to the animals were collected. Fine needle aspiration cytology and impression smear were stained with Giemsa stain. Tissue samples were collected in 10 per cent neutral buffered formalin for histopathological examination. Special staining with Fontana silver stain and immunohistochemistry with Melan- A and S- 100 markers for confirmatory diagnosis of melanin pigment was done. Result: Cytology revealed neoplastic cells with vacuolation and pleomorphism. Cytoplasm contained abundant melanin pigments. Microscopically, the cytoplasm contained abundant brown, black coloured melanin pigment granules. Tissue sections stained with Fontana silver impregnation method revealed the presence of black coloured granules. Immunohistochemistry with Melan-A and S-100 revealed strong expression of brown coloured reaction in the cytoplasm of the neoplastic cells.

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对印度泰米尔纳德邦纳玛卡尔及其周边地区自然发生的牛皮肤黑色素瘤的细胞学、组织病理学和免疫组织化学研究

背景：黑色素瘤是源于黑色素母细胞的肿瘤。由于一些黑色素细胞肿瘤是先天性的，而且多发于幼畜，因此对牛肿瘤的研究是临床关注的焦点。本研究旨在研究牛黑色素瘤的病理形态特征，以便早期诊断和干预。研究方法在研究期间，从四头动物的疑似肿瘤病例中采集肿瘤样本。收集动物的相关数据。细针穿刺细胞学检查和印模涂片均用吉氏染色法染色。在 10% 中性缓冲福尔马林中采集组织样本，进行组织病理学检查。用丰塔纳银染色法进行特殊染色，并用 Melan- A 和 S- 100 标记进行免疫组化，以确诊黑色素沉着。结果：细胞学检查发现肿瘤细胞空泡化和多形性。细胞质中含有丰富的黑色素。显微镜下，细胞质中含有大量棕色、黑色的黑色素颗粒。用丰塔纳银浸渍法染色的组织切片显示存在黑色颗粒。用 Melan-A 和 S-100 进行免疫组化检查发现，肿瘤细胞的细胞质中强烈表达棕色反应。

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来源期刊

Indian Journal of Animal Research AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

1.00

自引率

20.00%

发文量

332

审稿时长

6 months

期刊介绍： The IJAR, the flagship print journal of ARCC, it is a monthly journal published without any break since 1966. The overall aim of the journal is to promote the professional development of its readers, researchers and scientists around the world. Indian Journal of Animal Research is peer-reviewed journal and has gained recognition for its high standard in the academic world. It anatomy, nutrition, production, management, veterinary, fisheries, zoology etc. The objective of the journal is to provide a forum to the scientific community to publish their research findings and also to open new vistas for further research. The journal is being covered under international indexing and abstracting services.