Individual and Dual Cytotoxicity of the Combination of Passiflora Caerulea Leaf Extract and Titanium Oxide Nanoparticles against A549, U937, and Hela Cells

Q2 Pharmacology, Toxicology and Pharmaceutics

Current Bioactive Compounds Pub Date : 2024-03-19 DOI:10.2174/0115734072294581240311064402

L. Sindhura, Mohd. Nazneen Bobby, K. Srikanth, Helan Soundra Rani Michael, Abu Md Ashif Ikbal, Sabu Thomas, H. Kargarzadeh, Partha Palit

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Abstract

The study addresses the underexplored realm of cytotoxicity evaluation involving binary mixtures of Passiflora caerulea leaf extracts and Titanium nanoparticles (TiO2NPs). The focus lies on understanding the combined effects of these components on cancer cells (A549, U937, and HeLa). In vitro cytotoxicity assessments were employed to evaluate the toxicity of P. caerulea leaf extracts, TiO2NPs, and their combination. The study utilized MTT, NRU, and LDH assays to measure cellular viability. Additionally, reactive oxygen species (ROS) and glutathione levels were assessed alongside the aforementioned assays. The toxicity percentage exhibited dose-dependent behavior for P. caerulea leaf extracts, TiO2NPs, and their combination. Interestingly, when P. caerulea leaf extract and TiO2NPs were combined, the reduction in cell viability was noticeably more than when the exposures were made separately. Moreover, the production of ROS was higher in the combined toxicity scenario, and a more pronounced decrease in glutathione levels was observed compared to individual exposures. The findings suggest that the combined effects of P. caerulea leaf extract and TiO2NPs induce greater cytotoxicity compared to their impacts. This underscores the potential for synergistic cytotoxicity in combined exposure scenarios, warranting further exploration of combined effects in future studies.

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西番莲叶提取物和纳米氧化钛组合对 A549、U937 和 Hela 细胞的单独和双重细胞毒性

该研究涉及西番莲叶提取物和钛纳米粒子（TiO2NPs）二元混合物的细胞毒性评估这一尚未充分开发的领域。研究重点在于了解这些成分对癌症细胞（A549、U937 和 HeLa）的综合影响。体外细胞毒性评估用于评价西番莲叶提取物、TiO2NPs 及其组合的毒性。该研究利用 MTT、NRU 和 LDH 检测法测量细胞活力。对于 P. caerulea 叶提取物、TiO2NPs 和它们的组合，毒性百分比表现出剂量依赖性。有趣的是，当 P. caerulea 叶提取物和 TiO2NPs 混合使用时，细胞活力的降低幅度明显高于单独使用时。此外，与单独接触相比，在联合毒性情况下产生的 ROS 更高，谷胱甘肽水平的下降也更明显。这强调了在联合暴露情况下产生协同细胞毒性的可能性，值得在今后的研究中进一步探讨联合效应。

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来源期刊

Current Bioactive Compounds Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

1.90

自引率

0.00%

发文量

112

期刊介绍： The journal aims to provide comprehensive review articles on new bioactive compounds with proven activities in various biological screenings and pharmacological models with a special emphasis on stereoeselective synthesis. The aim is to provide a valuable information source of bioactive compounds synthesized or isolated, which can be used for further development of pharmaceuticals by industry and academia. The journal should prove to be essential reading for pharmacologists, natural product chemists and medicinal chemists who wish to be kept informed and up-to-date with the most important developments on new bioactive compounds of natural or synthetic origin, including their stereoeselective synthesis.