Rikkunshito increases peripheral incretin-hormone levels in humans and rats

Hiroshi Kono, S. Furuya, H. Akaike, K. Shoda, Y. Kawaguchi, H. Amemiya, Hiromichi Kawaida, D. Ichikawa
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Abstract

BACKGROUND It was reported that rikkunshito (TJ-43) improved the cisplatin-induced decreases in the active form of ghrelin in plasma; however, other effects on gastrointestinal hormones have not been investigated. AIM To investigate the effects of TJ-43 on peripheral levels of incretin hormones, including gastric inhibitory polypeptide (GIP) and glucagon-like polypeptide-1 (GLP-1), in humans and rats. METHODS Patients were divided into two groups, namely patients who received TJ-43 immediately following surgery [TJ-43(+) group] and those who received TJ-43 on postoperative day 21 [TJ-43(-) group], and the plasma levels of active GIP and active GLP-1 were assessed. In animal experiments, rats were treated with TJ-43 [rat (r)TJ-43(+) group] or without [rTJ-43(−) group] by gavage for 4 wk, and the plasma active GIP and active GLP-1 levels were measured. The expression of incretin hormones in the gastrointestinal tract and insulin in the pancreas were investigated by immunohistochemistry. Furthermore, the cyclic adenosine monophosphate activities were assessed in pancreatic tissues from rats treated with or without TJ-43 in vivo , and the blood glucose levels and plasma insulin levels were measured in rats treated with or without TJ-43 in oral glucose tolerance tests. RESULTS In humans, the active incretin hormone levels increased, and values were significantly greater in the TJ-43(+) group compared those in the TJ-43(-) group. In rats, the plasma active incretin levels significantly increased in the rTJ-43(+) group compared with those in the rTJ-43(-) group. GIP and GLP-1 expressions were enhanced by TJ-43 treatment. Moreover, plasma insulin levels increased and blood glucose levels were blunted in the rTJ-43(+) group. CONCLUSION The results show that TJ-43 may be beneficial for patients who undergo pancreatic surgery.
利口酒能提高人类和大鼠外周增量激素的水平
背景 据报道,里坤实多(TJ-43)可改善顺铂诱导的血浆中胃泌素活性形式的下降,但尚未研究其对胃肠激素的其他影响。目的 研究 TJ-43 对人类和大鼠外周增量激素水平的影响,包括胃抑制多肽(GIP)和胰高血糖素样多肽-1(GLP-1)。方法 将患者分为两组,即术后立即接受 TJ-43 的患者[TJ-43(+)组]和术后第 21 天接受 TJ-43 的患者[TJ-43(-)组],并评估血浆中活性 GIP 和活性 GLP-1 的水平。在动物实验中,大鼠灌胃 TJ-43 [大鼠 (r)TJ-43(+) 组] 或不灌胃 TJ-43 [rTJ-43(-)组] 4 周,并测定血浆中活性 GIP 和活性 GLP-1 的水平。免疫组化法检测了胃肠道增量激素和胰腺中胰岛素的表达。此外,还评估了体内使用或不使用 TJ-43 的大鼠胰腺组织的环磷酸腺苷活性,并在口服葡萄糖耐量试验中测量了使用或不使用 TJ-43 的大鼠的血糖水平和血浆胰岛素水平。结果 在人体内,活性增量素激素水平升高,TJ-43(+)组的值明显高于 TJ-43(-)组。在大鼠中,与 rTJ-43(-)组相比,rTJ-43(+)组的血浆活性增量素水平明显增加。TJ-43治疗增强了GIP和GLP-1的表达。此外,rTJ-43(+)组血浆胰岛素水平升高,血糖水平降低。结论 结果表明,TJ-43 可能对接受胰腺手术的患者有益。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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