Antibacterial activity of endophytic fungi associated with Aspidosperma subincanum Mart. against Gram-positive bacteria

Angélica Diniz De Andrade, Ana Paula Gonçalves Coelho Vieira, Priscilla Rodrigues Valadares Campana, C. Silva, Vera Lúcia de Almeida, Andréia Fonseca Silva, D. O. Scoaris
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Abstract

Endophytic fungi are microorganisms that inhabit plant tissues, without causing damage to the host. When in association with medicinal plants, they are able to prompt the production of secondary metabolites with important biological activities. The aim of this study was to evaluate the antibacterial activity of endophytic fungi associated with Aspidosperma subincanum against Gram positive bacteria of clinical relevance and perform the chemical characterization of the extract with the highest antibacterial activity. Endophytic fungi isolated from leaves of A. subincanum were submitted to ethanolic extraction. The antibacterial activity of the extracts was evaluated against S. aureus ATCC 25923, using the microdilution method. From a total of 116 extracts, only 4 (3.44%) were considered positive and then evaluated against B. cereus. The ethanolic endophytic extract AM59.1-98 was able to completely inhibit the growth of both microorganisms and was selected for chemical characterization, which indicated the presence of saponins and tannins. Further studies have to be conducted to confirm if the chemical substances detected are those responsible for its antibacterial activity. The current research provides, for the first time, substantial results concerning the antibacterial activity of endophytes associated with A. subincanum as an alternative path in the search for new drugs.
与 Aspidosperma subincanum Mart. 相关的内生真菌对革兰氏阳性菌的抗菌活性
内生真菌是栖息在植物组织中的微生物,不会对宿主造成损害。当它们与药用植物结合时,能够促使产生具有重要生物活性的次级代谢产物。本研究旨在评估与 Aspidosperma subincanum 相关的内生真菌对临床相关革兰氏阳性细菌的抗菌活性,并对抗菌活性最高的提取物进行化学鉴定。从Aspidosperma subincanum叶片中分离出的内生真菌经过乙醇提取。采用微量稀释法评估了萃取物对金黄色葡萄球菌 ATCC 25923 的抗菌活性。在总共 116 种提取物中,只有 4 种(3.44%)被认为是阳性的,然后对蜡样芽孢杆菌进行了评估。乙醇内生提取物 AM59.1-98 能够完全抑制这两种微生物的生长,并被选中进行化学鉴定,结果表明其中含有皂苷和单宁酸。要确认检测到的化学物质是否是其抗菌活性的原因,还需要进行进一步的研究。目前的研究首次提供了与亚金仙草相关的内生菌抗菌活性的实质性结果,为寻找新药提供了另一条途径。
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