miR-155 and miR-223 as markers of biological and clinical features of chronic lymphocytic leukemia

Q4 Medicine
M. Perepechaeva, O. Goreva, O. Berezina, T. I. Pospelova, A. Grishanova
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引用次数: 0

Abstract

Introduction. Chronic lymphocytic leukemia (CLL) is a disease characterized by large individual differences both in the clinical course and in molecular patterns of expression of genes and regulatory RNAs, which can influence pathological changes. The involvement of regulatory microRNAs miR-155 and miR-223 in the pathogenesis of CLL is fairly well known, but there is insufficient information about possible fluctuations in the expression of miR-155 and miR-223 depending on the time course of pathology development and on parameters of medical treatment. Purpose – to investigate the expression of miR-155 and miR-223 in patients having CLL with different biological and clinical features and different characteristics of treatment in terms of peripheral-blood substrates (plasma, lymphocytes, and extracellular vesicles) and bone marrow. Material and Methods. This work involved samples of peripheral blood and bone marrow from 38 patients with a diagnosis of CLL from the City Hematology Center at the government-funded healthcare institution (Novosibirsk Oblast) City Clinical Hospital No. 2 from the years 2016 to 2017. Assessment of miR-155 and miR-223 expressions was carried out by reverse-transcription real-time PCR according to the TaqMan principle. Significance of differences between groups was evaluated either by the nonparametric Mann–Whitney test or by the nonparametric Kruskal–Wallis test with subsequent pairwise comparisons via the Mann–Whitney test. Results. High variation of the analyzed parameters was found. The expression levels of miR-155 and miR-223 in microvesicles of patients with unfavorable chromosomal anomalies were lower than those in patients with the chromosomal aberrations (or the normal karyotype) associated with a moderate effect on CLL prognosis. The expression level of miR-223 in peripheral blood lymphocytes of untreated patients with CLL was higher than that observed in treated patients. Conclusion. differences in the expression levels of miR-155 and miR-223 were identified depending on chromosomal aberrations and polychemotherapy. Our preliminary results will provide the basis for future larger studies on levels of microRNAs in CLL patients having specific features of the development, clinical course, and treatment of the disease.
miR-155 和 miR-223 作为慢性淋巴细胞白血病生物学和临床特征的标志物
简介慢性淋巴细胞白血病(CLL)是一种在临床病程以及基因和调控 RNA 分子表达模式方面都存在巨大个体差异的疾病,而这些差异会影响病理变化。众所周知,调控微RNA miR-155和miR-223参与了CLL的发病机制,但关于miR-155和miR-223的表达可能随病理发展的时间进程和药物治疗参数而波动的信息尚不充分。目的--从外周血基质(血浆、淋巴细胞和细胞外囊泡)和骨髓的角度,研究具有不同生物学和临床特征以及不同治疗特点的 CLL 患者体内 miR-155 和 miR-223 的表达情况。材料和方法。这项工作涉及政府资助医疗机构(新西伯利亚州)市第二临床医院市血液学中心 2016 年至 2017 年诊断为 CLL 的 38 名患者的外周血和骨髓样本。根据TaqMan原理,通过反转录实时PCR技术对miR-155和miR-223的表达进行了评估。组间差异的显著性通过非参数 Mann-Whitney 检验或非参数 Kruskal-Wallis 检验进行评估,随后通过 Mann-Whitney 检验进行配对比较。结果所分析的参数差异很大。染色体异常患者微囊中 miR-155 和 miR-223 的表达水平低于染色体畸变(或正常核型)患者,这对 CLL 预后有一定影响。未接受治疗的 CLL 患者外周血淋巴细胞中 miR-223 的表达水平高于接受治疗的患者。结论:miR-155 和 miR-223 的表达水平因染色体畸变和多化疗而异。我们的初步研究结果将为今后对具有疾病发展、临床过程和治疗特点的 CLL 患者的 microRNA 水平进行更大规模的研究奠定基础。
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来源期刊
Siberian journal of oncology
Siberian journal of oncology Medicine-Oncology
CiteScore
0.40
自引率
0.00%
发文量
117
审稿时长
8 weeks
期刊介绍: The main objectives of the journal are: -to promote the establishment of Russia’s leading worldwide positions in the field of experimental and clinical oncology- to create the international discussion platform intended to cover all aspects of basic and clinical cancer research, including carcinogenesis, molecular biology, epidemiology, cancer prevention, diagnosis and multimodality treatment (surgery, chemotherapy, radiation therapy, hormone therapy), anesthetic management, medical and social rehabilitation, palliative care as well as the improvement of life quality of cancer patients- to encourage promising young scientists to be actively involved in cancer research programs- to provide a platform for researches and doctors all over the world to promote, share, and discuss various new issues and developments in cancer related problems. (to create a communication platform for the expansion of cooperation between Russian and foreign professional associations).- to provide the information about the latest worldwide achievements in different fields of oncology The most important tasks of the journal are: -to encourage scientists to publish their research results- to offer a forum for active discussion on topics of major interest - to invite the most prominent Russian and foreign authors to share their latest research findings with cancer research community- to promote the exchange of research information, clinical experience, current trends and the recent developments in the field of oncology as well as to review interesting cases encountered by colleagues all over the world- to expand the editorial board and reviewers with the involvement of well-known Russian and foreign experts- to provide open access to full text articles- to include the journal into the international database- to increase the journal’s impact factor- to promote the journal to the International and Russian markets
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