Gelatin but not type I collagen promotes bacteria phagocytosis in PMA-treated U937 human lymphoma cells.

IF 2.8 4区 医学 Q3 CELL BIOLOGY
Connective Tissue Research Pub Date : 2024-03-01 Epub Date: 2024-03-25 DOI:10.1080/03008207.2024.2330693
Li Meiling, Chen Yiran, Sun Xiaoli, Chen Kaihui, Hayashi Toshihiko, Itoh Kikuji, Mizuno Kazunori, Shunji Hattori, Hitomi Fujisaki, Weiwei Liu, Takashi Ikejima
{"title":"Gelatin but not type I collagen promotes bacteria phagocytosis in PMA-treated U937 human lymphoma cells.","authors":"Li Meiling, Chen Yiran, Sun Xiaoli, Chen Kaihui, Hayashi Toshihiko, Itoh Kikuji, Mizuno Kazunori, Shunji Hattori, Hitomi Fujisaki, Weiwei Liu, Takashi Ikejima","doi":"10.1080/03008207.2024.2330693","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Besides comprising scaffolding, extracellular matrix components modulate many biological processes including inflammation and cell differentiation. We previously found precoating cell plates with extracellular matrix collagen I, or its denatured product gelatin, causes aggregation of macrophage-like human lymphoma U937 cells, which are induced to differentiation by phorbol myristate treatment. In the present study, we investigated the influence of gelatin or collagen I precoating on the bacteria phagocytosis in PMA-stimulated U937 cells.</p><p><strong>Materials and methods: </strong>Colony forming units of phagocytosed bacteria, Giemsa-staining of cells with phagocytosed bacteria, confocal microscopic and flow cytometric analysis of cells with phagocytosed FITC-labeled bacteria and non-bioactive latex beats were conducted.</p><p><strong>Results: </strong>Gelatin precoating enhances the phagocytosis of both Gram-negative and positive bacteria, as shown by the increased colony forming units of bacteria phagocytosed by cells, and increased intracellular bacteria observed after Giemsa-staining. But collagen I has no marked influence. Confocal microscopy reveals that both live and dead FITC-bacteria were phagocytosed more in the cells with gelatin-coating but not collagen-coating. Of note, both gelatin and collagen I coating had no influence on the phagocytosis of non-bioactive latex beads. Since gelatin-coating increases autophagy but collagen I has no such impact, we are curious about the role of autophagy. Inhibiting autophagy reduced the phagocytosis of bacteria, in cells with gelatin-coating, while stimulating autophagy enhanced phagocytosis.</p><p><strong>Conclusion: </strong>This study finds the bacteria-phagocytosis stimulatory effect of gelatin in PMA-treated U937 cells and reveals the positive regulatory role of autophagy, predicting the potential use of gelatin products in anti-bacterial therapy.</p>","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"170-185"},"PeriodicalIF":2.8000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Connective Tissue Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/03008207.2024.2330693","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/25 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Purpose: Besides comprising scaffolding, extracellular matrix components modulate many biological processes including inflammation and cell differentiation. We previously found precoating cell plates with extracellular matrix collagen I, or its denatured product gelatin, causes aggregation of macrophage-like human lymphoma U937 cells, which are induced to differentiation by phorbol myristate treatment. In the present study, we investigated the influence of gelatin or collagen I precoating on the bacteria phagocytosis in PMA-stimulated U937 cells.

Materials and methods: Colony forming units of phagocytosed bacteria, Giemsa-staining of cells with phagocytosed bacteria, confocal microscopic and flow cytometric analysis of cells with phagocytosed FITC-labeled bacteria and non-bioactive latex beats were conducted.

Results: Gelatin precoating enhances the phagocytosis of both Gram-negative and positive bacteria, as shown by the increased colony forming units of bacteria phagocytosed by cells, and increased intracellular bacteria observed after Giemsa-staining. But collagen I has no marked influence. Confocal microscopy reveals that both live and dead FITC-bacteria were phagocytosed more in the cells with gelatin-coating but not collagen-coating. Of note, both gelatin and collagen I coating had no influence on the phagocytosis of non-bioactive latex beads. Since gelatin-coating increases autophagy but collagen I has no such impact, we are curious about the role of autophagy. Inhibiting autophagy reduced the phagocytosis of bacteria, in cells with gelatin-coating, while stimulating autophagy enhanced phagocytosis.

Conclusion: This study finds the bacteria-phagocytosis stimulatory effect of gelatin in PMA-treated U937 cells and reveals the positive regulatory role of autophagy, predicting the potential use of gelatin products in anti-bacterial therapy.

明胶(而非 I 型胶原蛋白)能促进经 PMA 处理的 U937 人类淋巴瘤细胞吞噬细菌。
目的:细胞外基质成分除了作为支架外,还能调节许多生物过程,包括炎症和细胞分化。我们之前发现,用细胞外基质胶原蛋白 I 或其变性产物明胶预涂细胞板会导致巨噬细胞样人淋巴瘤 U937 细胞聚集,而这些细胞会在肉豆蔻酸薄荷酯的诱导下分化。在本研究中,我们研究了明胶或胶原蛋白 I 预涂层对 PMA 刺激的 U937 细胞吞噬细菌的影响:对吞噬细菌的菌落形成单位、吞噬细菌细胞的革兰氏染色、吞噬FITC标记细菌和无生物活性乳胶搏动的细胞进行共聚焦显微镜和流式细胞分析:结果:明胶预涂布增强了对革兰氏阴性和阳性细菌的吞噬能力,这表现在细胞吞噬细菌的菌落形成单位增加,以及 Giemsa 染色后观察到细胞内细菌增加。但胶原蛋白 I 没有明显的影响。共聚焦显微镜显示,在涂有明胶而非胶原蛋白的细胞中,活的和死的 FITC 细菌都被吞噬得更多。值得注意的是,明胶和胶原 I 包被对非生物活性乳胶珠的吞噬作用没有影响。由于明胶涂层会增加自噬,而胶原蛋白 I 却没有这种影响,我们对自噬的作用感到好奇。在涂有明胶的细胞中,抑制自噬会减少对细菌的吞噬,而刺激自噬则会增强吞噬能力:本研究发现了明胶在 PMA 处理的 U937 细胞中的细菌吞噬刺激作用,并揭示了自噬的积极调节作用,预测了明胶产品在抗菌治疗中的潜在用途。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Connective Tissue Research
Connective Tissue Research 生物-细胞生物学
CiteScore
6.60
自引率
3.40%
发文量
37
审稿时长
2 months
期刊介绍: The aim of Connective Tissue Research is to present original and significant research in all basic areas of connective tissue and matrix biology. The journal also provides topical reviews and, on occasion, the proceedings of conferences in areas of special interest at which original work is presented. The journal supports an interdisciplinary approach; we present a variety of perspectives from different disciplines, including Biochemistry Cell and Molecular Biology Immunology Structural Biology Biophysics Biomechanics Regenerative Medicine The interests of the Editorial Board are to understand, mechanistically, the structure-function relationships in connective tissue extracellular matrix, and its associated cells, through interpretation of sophisticated experimentation using state-of-the-art technologies that include molecular genetics, imaging, immunology, biomechanics and tissue engineering.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信