Effect of Water-Soluble Coronatine on Production of Paclitaxel in a Plant Cell Culture with Water-immiscible 1-Butyl-1-methylpyrrolidinium Bis(trifluoromethanesulfonyl)imide

Abstract

We report here the effect of a water-soluble elicitor, coronatine, on the amount of paclitaxel produced in a plant cell culture by in situ extraction with a water-immiscible ionic liquid, 1-butyl-1-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide (P14TFSI). The amount of paclitaxel produced in the cell culture without P14TFSI enhanced with increase of coronatine concentration, however, the amount of cell growth including coronatine was lower than that of the control because of feedback inhibition by the produced paclitaxel. The amount of the cell growth in the culture with P14TFSI and coronatine increased more than that in the control culture because the produced paclitaxel was decreased by in situ extraction with P14TFSI. The production amount of paclitaxel in the two-phase culture increased with increase of coronatine concentration, reached the peak in that including 1 µM coronatine and decreased. The production amount of paclitaxel in the culture including P14TFSI and 1 µM coronatine was 250 times greater than that of the control culture, possibly due to the synergistic effects of coronatine and abiotic stress of P14TFSI.