Genomic and Metagenomic Insights into the Distribution of Nicotine-Degrading Enzymes in Human Microbiota

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-21 DOI:10.2174/0113892029302230240319042208

Ying Guan, Zhouhai Zhu, Qiyuan Peng, Meng Li, Xuan Li, Jia-Wei Yang, Yan-Hong Lu, Meng Wang, Bin-Bin Xie

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Abstract

Introduction: Nicotine degradation is a new strategy to block nicotine-induced pathology. The potential of human microbiota to degrade nicotine has not been explored. Aims: This study aimed to uncover the genomic potentials of human microbiota to degrade nicotine. Method: To address this issue, we performed a systematic annotation of Nicotine-Degrading Enzymes (NDEs) from genomes and metagenomes of human microbiota. A total of 26,295 genomes and 1,596 metagenomes for human microbiota were downloaded from public databases and five types of NDEs were annotated with a custom pipeline. We found 959 NdhB, 785 NdhL, 987 NicX, three NicA1, and three NicA2 homologs. Results: Genomic classification revealed that six phylum-level taxa, including Proteobacteria, Firmicutes, Firmicutes_A, Bacteroidota, Actinobacteriota, and Chloroflexota, can produce NDEs, with Proteobacteria encoding all five types of NDEs studied. Analysis of NicX prevalence revealed differences among body sites. NicX homologs were found in gut and oral samples with a high prevalence but not found in lung samples. NicX was found in samples from both smokers and non-smokers, though the prevalence might be different.

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人类微生物群中尼古丁降解酶分布的基因组学和元基因组学启示

介绍：尼古丁降解是阻止尼古丁诱发病理学的一种新策略。人类微生物群降解尼古丁的潜力尚未得到探索。目的：本研究旨在揭示人类微生物群降解尼古丁的基因组潜力。方法：为了解决这个问题，我们对人类微生物群基因组和元基因组中的尼古丁降解酶（NDEs）进行了系统注释。我们从公共数据库中下载了26,295个人类微生物群的基因组和1,596个元基因组，并使用定制管道注释了五种类型的尼古丁降解酶。我们发现了 959 个 NdhB、785 个 NdhL、987 个 NicX、3 个 NicA1 和 3 个 NicA2 同源物。结果基因组分类显示，六个门级类群，包括变形菌门、固形菌门、固形菌_A 门、类杆菌门、放线菌门和绿藻门，都能产生 NDEs，其中变形菌门编码了所研究的所有五种 NDEs。对 NicX 流行情况的分析表明，不同的身体部位存在差异。在肠道和口腔样本中发现的 NicX 同源物的流行率很高，但在肺部样本中却没有发现。在吸烟者和非吸烟者的样本中都发现了 NicX，但流行率可能有所不同。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.